Anti-FXYD1 (Phospholemman) Rabbit Polyclonal Antibody for WB, ELISA - O00168

Core Product Specifications and Parameters

Biological Background: FXYD1 (Phospholemman) Function and Localization

• FXYD1, also known as Phospholemman, is a member of the FXYD domain-containing ion transport regulator family and acts as a regulatory subunit of the sodium/potassium-transporting ATPase (NKA).
• It modulates NKA activity in a phosphorylation-dependent manner: in its unphosphorylated state, FXYD1 inhibits NKA, while phosphorylation stimulates pump activity, thereby regulating cellular ion gradients.
• FXYD1 reduces glutathionylation of the NKA beta-1 subunit ATP1B1, thus reversing glutathionylation-mediated inhibition of NKA and maintaining proper pump function under oxidative stress.
• Beyond ion homeostasis, FXYD1 contributes to female sexual development by maintaining the excitability of gonadotropin-releasing hormone-secreting neurons.
• Subcellularly, FXYD1 localizes to the cell membrane, sarcolemma, apical cell membrane, caveolae, and T-tubules, reflecting its critical roles in excitable and epithelial tissues.
• Post-translational modifications including phosphorylation, palmitoylation, and glutathionylation are essential for its regulatory functions and membrane targeting.
• Tissue expression is highest in skeletal muscle and heart, with moderate levels in brain, placenta, lung, liver, pancreas, and other organs, consistent with its broad role in ion transport.

Experimental Guidance and Technical Tips

• The immunogen covers the N-terminal region (aa 1-92) of human FXYD1; for western blotting, expect a band near 10 kDa and validate specificity using peptide competition or relevant knockout controls.
• For ELISA applications, the polyclonal nature offers broad epitope coverage; titrate the antibody and optimize coating conditions to achieve a robust signal-to-noise ratio.
• With confirmed cross-reactivity to mouse and rat, this antibody is suitable for rodent model studies; always validate in the appropriate tissue or cell lysates to mitigate species-specific background.
• Note that FXYD1 phosphorylation states may affect antibody binding; include phosphatase inhibitors during sample preparation and consider using phospho‑specific methods for quantitative analysis.

CamelBio: Your One-Stop Sourcing Bridge

As a dedicated one-stop supplier of IVD raw materials, CamelBio equips diagnostic manufacturers and research teams with validated antibodies, bulk ancillary reagents, and rare target sourcing—supporting projects from early concept to clinic. For FXYD1/Phospholemman-focused work, we provide optimized polyclonal and monoclonal antibodies, technical consultation, and reliable supply chains to streamline your assay development in ion transport and cardiovascular research.