Anti-FXR2 Rabbit Polyclonal Antibody for WB, ELISA - P51116

Core Product Specifications and Parameters

Biological Background: FXR2 Function and Localization

• FXR2 (Fragile X mental retardation syndrome-related protein 2) is an RNA-binding protein belonging to the FXR family, which includes FMR1, FXR1, and FXR2. It is also known as FMR1 autosomal homolog 2.
• It functions as a regulator of mRNA translation and stability by specifically binding to AU-rich elements (AREs) in the 3'-UTR of target mRNAs (By similarity).
• FXR2 promotes the formation of phase-separated membraneless compartments through liquid-liquid phase separation upon binding ARE-containing mRNAs, thereby modulating mRNA storage and translation (By similarity).
• The protein is required for adult hippocampal neurogenesis, where it regulates the translation and/or stability of NOG (noggin) mRNA to prevent NOG protein expression in the dentate gyrus (By similarity).
• Subcellularly, FXR2 localizes to the cytoplasm, cytoplasmic ribonucleoprotein granules, and the postsynaptic region.
• It is widely expressed in tissues including heart, brain, kidney, and testis. In the brain, FXR2 is present at high levels in neurons, particularly in Purkinje cells at the interface between the granular and molecular layers. Related references: PMID:9259278
• Post-translational modifications include phosphorylation, and the protein contains RNA-binding repeat domains. It is classified under keywords such as Phosphoprotein and Synapse.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide spanning amino acids 550-650 of human FXR2 (NP_004851.2). When using this antibody in non-human species, verify sequence homology across the epitope region to confirm cross-reactivity, as it is reported to detect mouse and rat FXR2.
• For Western blot, expect a band at the reported molecular weight of approximately 74 kDa. Optimize blocking and detection conditions for your specific sample type.
• This antibody is suitable for ELISA applications. Consider pairing it with a validated secondary antibody and optimize coating antigen concentrations for your assay.
• Since FXR2 functions in RNA-binding and phase separation, gentle lysis conditions may be required to preserve native interactions when studying RNA-binding properties.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your comprehensive partner for IVD raw material sourcing, offering validated antibody pairs, monoclonal and polyclonal antibodies, and ancillary reagents. For targets like FXR2, involved in neurogenesis and mRNA regulation, we provide one-stop access to high-quality antibodies suitable for diagnostic and research applications. Let CamelBio bridge the gap from concept to clinic with reliable, scalable solutions.