Anti-FXR2 Polyclonal Antibody for WB, IF/ICC, ELISA - P51116

Core Product Specifications and Parameters

Biological Background: FXR2 Function and Localization

• RNA-binding protein that acts as a regulator of mRNA translation and/or stability, and which is required for adult hippocampal neurogenesis.
• Specifically binds to AU-rich elements (AREs) in the 3'-UTR of target mRNAs.
• Promotes formation of phase-separated membraneless compartments by undergoing liquid-liquid phase separation upon binding to AREs-containing mRNAs, thereby regulating mRNA storage, translation and/or stability.
• Acts as a key regulator of adult hippocampal neurogenesis by controlling NOG mRNA translation/stability, preventing NOG protein expression in the dentate gyrus.
• Localizes to the cytoplasm, cytoplasmic ribonucleoprotein granules, and the postsynapse.
• Expressed in all tissues examined including heart, brain, kidney and testis. In brain, present at high level in neurons and especially in the Purkinje cells at the interface between the granular layer and the molecular layer. Related references: PMID:9259278
• Belongs to the FMR1 family and is also known as FMR1 autosomal homolog 2.
• Post-translational modifications include phosphorylation, as indicated by the Phosphoprotein keyword.

Experimental Guidance and Technical Tips

• For Western blot, expect a specific band around 74 kDa based on the predicted molecular weight. Validate the antibody in relevant cell or tissue lysates, such as neuronal cells or brain tissue.
• For immunofluorescence/ICC, use appropriate fixation and permeabilization protocols to detect cytoplasmic and granular localizations. Include proper negative controls to confirm specificity.
• For ELISA, the immunogen peptide can serve as a positive control. Consider including pre-immune serum as a negative control when using polyclonal antibodies.
• As a polyclonal antibody, recognize that lot-to-lot variability may occur. It is advisable to validate performance in your specific experimental context.
• Cross-reactivity with mouse and rat has been reported, making it suitable for studies across these species.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive, one-stop sourcing platform for IVD raw materials, supporting diagnostic manufacturers and research laboratories from discovery to clinical application. For FXR2-related studies in mRNA regulation and neurogenesis, we can provide validated antibody pairs, optimized polyclonal antibodies like this anti-FXR2 reagent, and bulk ancillary reagents to streamline your workflow. Our services extend to rare target raw-material sourcing, ensuring you obtain the specific tools needed with reliable supply chain support.