Anti-FXR1 Rabbit Polyclonal Antibody for WB and ELISA - P51114

Core Product Specifications and Parameters

Biological Background: FXR1 Function and Localization

• FXR1 is an RNA-binding protein that regulates mRNA translation and stability, essential for neurogenesis, muscle development, and spermatogenesis. Related references: PMID:17382880, PMID:20417602, PMID:30067974
• It specifically binds to AU-rich elements (AREs) in 3'-UTRs, undergoing liquid-liquid phase separation to form cytoplasmic ribonucleoprotein granules that concentrate mRNAs and regulatory factors. Related references: PMID:17382880, PMID:34731628
• FXR1 activates translation of stored mRNAs during spermatogenesis by assembling target mRNAs into granules that recruit EIF4G3, and also promotes MYC translation by recruiting the eIF4F complex. Related references: PMID:34731628
• Acts as a negative regulator of inflammation by destabilizing pro-inflammatory transcripts in response to IL19 and binding TNF mRNA to reduce TNF production. Related references: PMID:30067974
• In neurons, inhibits GRIA2/AMPA receptor synthesis during synaptic potentiation and regulates neural stem cell proliferation via CDKN1A mRNA, also contributing to sleep and synaptic homeostasis.
• Required for muscle development via phase-separated ribonucleoprotein granules, and aids nuclear pore complex localization by preventing nucleoporin aggregation. Related references: PMID:30770808, PMID:32706158
• Stabilizes PKP2 mRNA through interaction with PKP3, and forms cytoplasmic mRNP networks that scaffold kinases and substrates for signaling (e.g., actomyosin reorganization). Related references: PMID:25225333, PMID:39106863
• Subcellularly localizes to cytoplasm, stress granules, dendrites, dendritic spines, axons, nuclear envelope, and postsynapse. Widely expressed in heart, brain, kidney, testis, with high levels in Purkinje cells. Related references: PMID:7781595, PMID:9259278

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide spanning amino acids 250-350 of human FXR1 (NP_005078.2). Users may consider verifying reactivity with specific splice isoforms or paralogs if needed.
• For Western blotting, a band around 70 kDa is expected. Positive controls like HeLa, brain, or muscle lysates can be tested, given the reported expression in these tissues.
• Cross-reactivity with mouse and rat is indicated; however, validation in the relevant species is recommended.
• For ELISA, the peptide or recombinant FXR1 protein can be used as coating antigen. Optimization of antibody dilution and blocking conditions is advisable.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your dedicated partner for IVD raw materials, offering validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and custom sourcing for rare targets. This FXR1 polyclonal antibody supports research into mRNA regulation, neurodevelopment, and muscle biology. Explore our comprehensive catalog to accelerate your diagnostic development from concept to clinic.