Anti-FUT8 Polyclonal Antibody for WB, ELISA - Q9BYC5

Core Product Specifications and Parameters

Biological Background: FUT8 Function and Localization

• FUT8, encoded by the FUT8 gene, is an alpha-(1,6)-fucosyltransferase also known as CDGF or CDGF1.
• It catalyzes the addition of fucose in alpha 1-6 linkage to the first GlcNAc residue of N-glycans, a critical modification of glycoproteins including the Fc region of immunoglobulins. Related references: PMID:17172260, PMID:29304374, PMID:36280670
• FUT8 is a Golgi-resident type II membrane protein, localizing specifically to the Golgi stack membrane.
• It belongs to the glycosyltransferase family and features a signal-anchor, a transmembrane helix, and an SH3-binding domain.
• Mutations in FUT8 are associated with congenital disorder of glycosylation (CDG), highlighting its clinical significance.
• The enzyme undergoes phosphorylation and alternative splicing, events that may modulate its activity or isoform expression.

Experimental Guidance and Technical Tips

• The immunogen covers amino acids 376-575 of human FUT8, targeting the C-terminal region; consider whether this region may be present in alternative splicing isoforms and validate detection in your sample system.
• For Western blot, a band near 67 kDa is expected; use appropriate positive controls such as human or mouse cell lines expressing FUT8.
• Polyclonal nature may provide robust signal in ELISA; optimize coating and detection conditions for your assay format.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD raw materials, offering this anti-FUT8 polyclonal antibody to support research in glycosylation, immunoglobulin biology, and congenital disorders. Our platform delivers validated antibody pairs, optimized monoclonal/polyclonal reagents, and bulk ancillary materials to accelerate your assay development. With expertise in rare-target sourcing, CamelBio bridges the gap from concept to clinic.