Anti-FUT6 Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - P51993

Core Product Specifications and Parameters

Biological Background: FUT6 Function and Localization

• FUT6 (Fucosyltransferase 6), also designated Fuc-TVI, is a glycosyltransferase belonging to the alpha-1,3-fucosyltransferase family. It catalyzes the transfer of L-fucose from GDP-beta-L-fucose to the N-acetylglucosamine (GlcNAc) of acceptor substrates, participating in the biosynthesis of sialyl Lewis X (sLe(x)), Lewis X (Le(x)), and VIM2 determinants. Related references: PMID:10728707, PMID:1339443, PMID:1520296
• FUT6 exhibits specificity for distal alpha2,3-sialylated and neutral polylactosamine chains on glycoproteins and glycolipids. It can further fucosylate H-type 2 chain acceptors to generate difucosylated sialyl Lewis x structures but lacks activity toward type 1 and H-type 1 chain substrates. Related references: PMID:1339443, PMID:17604274, PMID:9363434
• At the subcellular level, FUT6 localizes primarily to the Golgi stack membrane, where it functions as a resident type II membrane protein, and is also found in secreted forms.
• Tissue expression profiling reveals FUT6 in kidney, liver, colon, small intestine, bladder, uterus, and salivary gland.
• The protein undergoes alternative splicing and is a glycoprotein with a single-pass transmembrane domain. It is a signal-anchor protein and belongs to the glycosyltransferase family.
• By synthesizing fucosylated carbohydrate epitopes such as sLe(x), FUT6 plays a role in cell adhesion, leukocyte trafficking, and inflammatory responses, making it a molecule of interest in cancer and immune research.

Experimental Guidance and Technical Tips

• The immunogen used to generate this antibody encompasses amino acids 170–359 of human FUT6, a region covering the catalytic domain. This design likely enables recognition of both membrane-bound and secreted forms of the enzyme.
• In Western blotting (WB), the predicted molecular weight is approximately 42 kDa; however, glycosylation may cause slight shifts. Validate antibody binding under reducing and non-reducing conditions in your relevant sample system.
• For immunohistochemistry (IHC-P), consider optimal antigen retrieval methods and include negative controls to distinguish specific staining, especially given the Golgi localization pattern.
• ELISA applications can benefit from titrating the antibody against the recombinant immunogen or native FUT6 to determine appropriate working concentrations.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic and research communities by providing streamlined access to high-quality IVD raw materials. For fucosyltransferase and glycobiology projects, we offer validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents. Reach out to explore how our sourcing capabilities can accelerate your development of sialyl Lewis X, Lewis antigen, or glycosylation-related assays.