Anti-FUT4 Rabbit Polyclonal Antibody for WB, IF-P, IHC-P, ELISA - P22083

Core Product Specifications and Parameters

Biological Background: FUT4 Function and Localization

• Encodes alpha-(1,3)-fucosyltransferase 4 (FUT4), a Golgi-resident transmembrane enzyme.
• Catalyzes the transfer of fucose from GDP-Beta-L-fucose to N-acetylglucosamine (GlcNAc) within type 2 lactosamine (LacNAc) to form the Lewis X antigen (CD15) (PubMed:1702034, PubMed:1716630, PubMed:29593094). Related references: PMID:1702034 PMID:1716630 PMID:29593094
• Also fucosylates sialylated LacNAc acceptors, generating sialyl Lewis X and 6-sulfo sialyl Lewis X determinants that serve as C-type lectin epitopes (PubMed:1716630, PubMed:29593094). Related references: PMID:1716630 PMID:29593094
• Cooperates with FUT7 to synthesize selectin ligands (e.g., SELE, SELL, SELP), critical for lymphocyte homing, leukocyte migration, and blood leukocyte homeostasis (By similarity).
• In a cell-type-specific context, can fucosylate internal LacNAc units of polylactosamine chains to produce VIM-2 antigen, a recognition determinant for SELE (PubMed:11278338, PubMed:1716630). Related references: PMID:11278338 PMID:1716630
• Localized to the Golgi stack membrane.
• Expressed in bone marrow-derived mesenchymal stem cells, cord blood immature promyelocytes, and peripheral blood myeloid and lymphoid populations.
• Classified under keywords: Alternative initiation, Glycoprotein, Glycosyltransferase, Golgi apparatus, Inflammatory response, Membrane, Signal-anchor, Transferase, Transmembrane helix.

Experimental Guidance and Technical Tips

• The immunogen corresponds to an internal region (aa 186-331) of human FUT4; thus the antibody detects an epitope likely conserved across species (cross-reacts with mouse and rat).
• For Western blotting, electrophorese samples under denaturing conditions and probe for a band at ~59 kDa. Consider using HL-60 or U937 cell lysates as positive controls.
• For immunofluorescence and immunohistochemistry, anticipate a Golgi-like perinuclear staining pattern. Use appropriate fixation and permeabilization conditions for organelle visualization.
• ELISA protocols may be optimized using recombinant FUT4 protein or peptide fragments as coating antigens.
• Validate antibody performance in your specific sample system and assay conditions before drawing conclusions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your integrated partner for IVD raw material procurement, offering validated antibody pairs, optimized polyclonal and monoclonal antibodies, and ancillary reagents—all in research and bulk quantities. Our portfolio includes rare-target-specific antibodies like this anti-FUT4 pAb, designed to support fucosyltransferase and glycobiology research. Whether you need ready-to-use detection antibodies or custom raw-material sourcing, CamelBio streamlines your development pipeline from concept to clinic.