Anti-FUT11 Rabbit Polyclonal Antibody for WB and ELISA - Q495W5

Core Product Specifications and Parameters

Biological Background: FUT11 Function and Localization

• FUT11, also known as POFUT4 or fucosyltransferase 11, is a GDP-fucose protein O-fucosyltransferase that catalyzes O-fucosylation of serine/threonine residues in EMI domains of target proteins, including MMRN1, MMRN2, and EMID1. Related references: PMID:39775168
• This modification likely facilitates protein folding and secretion of EMI domain-containing proteins. Related references: PMID:39775168
• Additionally, FUT11 exhibits minor alpha-(1,3)-fucosyltransferase activity toward biantennary N-glycan acceptors, though the in vivo relevance remains unclear. Related references: PMID:19088067
• The enzyme is localized to the endoplasmic reticulum membrane, consistent with its role in early glycosylation events.
• FUT11 contains a transmembrane helix and signal-anchor, anchoring it to the ER membrane.
• The protein is subject to alternative splicing, which may generate multiple isoforms with potentially distinct functions or localizations.
• FUT11 contains disulfide bonds and is itself a glycoprotein, as indicated by proteomics identification.

Experimental Guidance and Technical Tips

• For Western blotting, use a whole-cell lysate or microsomal fraction from human cell lines to detect the ~56 kDa band corresponding to FUT11; consider including a positive control expressing recombinant FUT11.
• Due to alternative splicing, multiple bands may be observed; validate antibody specificity using siRNA or knockout controls.
• For ELISA, this polyclonal antibody can be employed as capture or detection reagent; optimize coating conditions and evaluate cross-reactivity with closely related fucosyltransferases.
• Since the immunogen covers amino acids 25-205, the antibody may recognize the N-terminal cytoplasmic and partial stem region; confirm reactivity with full-length endogenous protein.
• Use standard blocking buffers (e.g., 5% non-fat milk or BSA) and avoid harsh stripping conditions if re-probing is required.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with one-stop access to premium IVD raw materials, technical services, and consulting—from concept to clinic. Our anti-FUT11 polyclonal antibody, validated for WB and ELISA, supports glycobiology research into O-fucosylation pathways and congenital disorders of glycosylation. Beyond individual antibodies, we offer validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare-target sourcing solutions to streamline your assay development and scale-up.