Anti-FUS Rabbit Polyclonal Antibody for WB, IHC-P, IP, ELISA - P35637

Core Product Specifications and Parameters

Biological Background: FUS Function and Localization

• RNA-binding protein FUS (also designated TLS) is a multifunctional DNA/RNA-binding protein involved in transcription regulation, RNA splicing, RNA transport, and DNA damage repair. Related references: PMID:27731383
• Binds specifically to ssRNA with the consensus sequence 5'-AGGUAA-3' and to nascent pre-mRNAs, mediating the coupling of transcription and splicing via RNA polymerase II and U1 snRNP. Related references: PMID:21256132, PMID:26124092
• Autoregulates its own expression through binding to its pre-mRNA, a mechanism mediated by nonsense-mediated decay. Related references: PMID:24204307
• Facilitates DNA double-strand break repair by promoting D-loop formation and homologous recombination. Related references: PMID:10567410
• In neuronal cells, plays critical roles in dendritic spine formation and stability, RNA transport, mRNA stability, and synaptic homeostasis.
• Subcellular localization is predominantly nuclear.
• Ubiquitously expressed; the protein features zinc finger and RNA recognition motifs, undergoes post-translational modifications including phosphorylation, methylation, and ubiquitination, and is associated with amyotrophic lateral sclerosis and oncogenic rearrangements.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 297–526 within the C-terminal region of human FUS, which includes the zinc finger domain and a portion of the RNA recognition motifs.
• This rabbit polyclonal antibody detects endogenous FUS in multiple applications: Western blot (expected band ~53 kDa), immunohistochemistry on paraffin-embedded sections, immunoprecipitation, and ELISA.
• Reported cross-reactivity with mouse and rat FUS; however, due to potential species-specific variations, validation in the target sample system is recommended.
• For IHC-P, consider antigen retrieval optimization and positive control tissues such as brain or testis.
• As FUS is subject to post-translational modifications, additional bands may appear in some cell lines; verify band patterns with appropriate controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a one‑stop supply solution for IVD raw materials, supporting diagnostic developers with validated antibody pairs, high‑quality monoclonal and polyclonal antibodies, and bulk ancillary reagents. For FUS‑related research in neurodegeneration and oncology, we offer ready‑to‑use antibodies and custom sourcing of rare targets to accelerate your assay development from concept to clinical application.