Anti-FUS (KO Validated) Polyclonal Antibody for WB, IF/ICC, IP, ELISA - P35637

Core Product Specifications and Parameters

Biological Background: FUS Function and Localization

• Multifunctional DNA/RNA-binding protein involved in transcription regulation, RNA splicing, RNA transport, DNA repair, and damage response (PubMed:27731383).
  Related references:
  PMID:27731383
• Binds specifically to single-stranded RNA containing the consensus sequence 5'-AGGUAA-3' (PubMed:21256132).
  Related references:
  PMID:21256132
• Acts as a molecular mediator between RNA polymerase II and U1 snRNP, coupling transcription with pre-mRNA splicing (PubMed:26124092).
  Related references:
  PMID:26124092
• Autoregulates its own expression by binding its pre-mRNA and triggering nonsense-mediated decay (PubMed:24204307).
  Related references:
  PMID:24204307
• Promotes D-loop formation and homologous recombination during DNA double-strand break repair (PubMed:10567410).
  Related references:
  PMID:10567410
• Predominantly localized in the nucleus where it exerts its regulatory functions.
• Ubiquitously expressed across a wide range of human tissues.
• Implicated in amyotrophic lateral sclerosis (ALS) and other neurodegenerative diseases; chromosomal rearrangements involving FUS are associated with certain cancers.

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 297–526 of human FUS (NP_004951.1), covering the C-terminal RNA recognition and zinc finger domains; the resulting polyclonal antibody recognizes multiple epitopes and is suitable for Western blot, immunofluorescence, immunoprecipitation, and ELISA.
• For Western blotting, FUS migrates at an apparent molecular weight of ~53 kDa; use appropriate reducing conditions and validate with a knockout control where available.
• In immunofluorescence/ICC, permeabilization is required; optimize fixation and blocking conditions for your cell type to reduce non-specific nuclear staining.
• This antibody has been shown to cross-react with mouse and rat FUS; verify specificity in your model system using appropriate tissue extracts or cell lysates.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD raw materials, providing diagnostic manufacturers and research institutions with validated antibodies, recombinant proteins, and ancillary reagents. The anti-FUS polyclonal antibody supports research into RNA biology, DNA repair mechanisms, and neurodegenerative disease pathways. For assay development or large‑scale procurement, our team offers tailored solutions, including optimized antibody pairs and bulk‑order logistics, to accelerate your projects from concept to clinic.