Anti-Furin Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - P09958

Core Product Specifications and Parameters

Biological Background: Furin Function and Localization

• Furin, also known as PACE, PCSK3, or SPC1, is a member of the proprotein convertase family encoded by the FURIN gene.
• Functions as a ubiquitous endoprotease within constitutive secretory pathways, cleaving at the RX(K/R)R consensus motif (PubMed:11799113, PubMed:1629222, PubMed:1713771). Related references: PMID:11799113, PMID:1629222, PMID:1713771
• Mediates proteolytic processing of TGFB1, an essential step for TGF-beta-1 activation (PubMed:7737999). Related references: PMID:7737999
• Converts the brain natriuretic factor prohormone into its active hormone BNP(1-32) through proteolytic cleavage (PubMed:20489134, PubMed:21763278). Related references: PMID:20489134, PMID:21763278
• Plays a role in host-pathogen interactions by cleaving and activating various bacterial and viral proteins, including diphtheria toxin, anthrax protective antigen, HIV-1 gp160, influenza hemagglutinin, and facilitating SARS-CoV-2 spike protein cleavage for cell entry.
• Localizes to the Golgi apparatus, trans-Golgi network membrane, cell membrane, endosome membrane, and can be secreted.
• Post-translationally modified: undergoes autocatalytic cleavage for zymogen activation, phosphorylation, glycosylation, and contains disulfide bonds; binds calcium and heparin.
• Ubiquitously expressed in human tissues.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a peptide within amino acids 200-300 of human Furin; consider that the antibody may preferentially recognize epitopes in this region.
• For Western blot, an expected band around 87 kDa is observed, but due to zymogen processing and autocatalytic cleavage, additional bands corresponding to pro- and mature forms may appear. Validate band patterns in appropriate cell lines or tissues.
• For immunofluorescence/ICC, optimized fixation and permeabilization conditions may be required to detect Golgi and TGN localization. Include appropriate controls.
• This polyclonal antibody is suitable for ELISA; recommend titrating to determine optimal working dilutions for specific assay conditions.
• The antibody cross-reacts with mouse and rat Furin, allowing use in those species; always validate in the relevant sample system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. As your sourcing partner for Furin research, we offer validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and support for rare target raw-material procurement.