Anti-Furin Monoclonal Antibody for WB and ELISA - P09958

Core Product Specifications and Parameters

Biological Background: Furin Function and Localization

• Furin (PCSK3) is a calcium-dependent serine endoprotease belonging to the proprotein convertase family, also known as paired basic amino acid residue-cleaving enzyme (PACE) and dibasic-processing enzyme.
• Furin recognizes and cleaves polypeptide precursors at the consensus sequence RX(K/R)R, playing a critical role in proprotein maturation within the constitutive secretory pathway. Related references: PMID:11799113, PMID:1629222, PMID:1713771
• It processes key proteins such as transforming growth factor beta-1 (TGFB1) and brain natriuretic factor prohormone, converting them into their active forms. Related references: PMID:7737999, PMID:20489134, PMID:21763278
• Furin cycles between the trans-Golgi network, cell surface, and endosomes, and is also secreted in a soluble form.
• The protein undergoes autocatalytic cleavage to generate the active enzyme, and contains disulfide bonds, glycosylation, and phosphorylation sites that regulate its function.
• Furin is ubiquitously expressed in human tissues, reflecting its central role in processing a wide array of secreted and membrane proteins.
• Furin is exploited by numerous pathogens to activate their surface proteins, including HIV-1 gp160, influenza hemagglutinin, and SARS-CoV-2 spike protein, making it a target for antiviral research.
• By processing the accessory subunit ATP6AP1 of V-ATPase, Furin indirectly regulates the acidification of secretory granules in pancreatic islet cells.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide from amino acids 200–300; the antibody likely recognizes a linear epitope, making it suitable for denaturing applications such as Western blot. For native applications like ELISA, validate binding under non-denaturing conditions.
• In Western blot, expect a band around 87 kDa for full-length Furin. Due to proprotein processing, additional bands corresponding to the proform and mature cleaved forms may also appear.
• Ubiquitous expression allows many cell lines to serve as positive controls; consider HeLa, HEK293, or A549 cells. Cross-reactivity with mouse is indicated, but verify sequence homology in the immunogen region when testing other species.
• For ELISA, optimize coating conditions and antibody titers, as the peptide immunogen may influence recognition of the native protein.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting. Our anti-Furin monoclonal antibody supports research into proprotein processing, viral pathogenesis, and cancer biology. We offer validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare-target sourcing to accelerate your assay development from concept to clinic.