Anti-FUNDC1 Polyclonal Antibody for WB, IHC-P, ELISA - Q8IVP5

Core Product Specifications and Parameters

Biological Background: FUNDC1 Function and Localization

• FUNDC1 (FUN14 domain-containing protein 1) is a mitochondrial outer membrane protein that integrates autophagy and mitochondrial quality control.
• It mediates formation of mitochondria-associated ER membranes (MAMs) and promotes angiogenesis through regulation of intracellular Ca(2+) and VEGFR2 expression. Related references: PMID:33972548
• As a mitophagy receptor, FUNDC1 recruits LC3 family proteins to mitochondria during hypoxia, facilitating selective autophagic degradation. Related references: PMID:22267086 | PMID:24671035 | PMID:24746696
• It also interacts with DRP1 at ER-mitochondria contact sites, driving mitochondrial fission under hypoxic conditions. Related references: PMID:27145933 | PMID:33978709
• Recent evidence shows FUNDC1 is involved in hepatic ferroptosis by binding to GPX4 and targeting it for mitophagic degradation. Related references: PMID:36828120
• Subcellularly, FUNDC1 is anchored in the mitochondrial outer membrane and is widely expressed in tissues.
• Post-translational modifications include phosphorylation and ubiquitin conjugation, as indicated by UniProt keywords.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide corresponding to the N-terminal region (aa 1-155) of human FUNDC1; this sequence is expected to detect full-length FUNDC1 and potentially any N-terminal isoforms.
• For Western blotting, the predicted molecular weight is 17 kDa; consider using reducing conditions and validate band detection in relevant human or mouse cell/tissue lysates.
• In IHC-P, antigen retrieval (e.g., citrate buffer pH 6.0) may be required; include appropriate positive and negative tissue controls.
• For ELISA, the antibody can be used for direct or indirect assays; titrate the antibody concentration for optimal signal-to-noise ratio.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a one-stop sourcing bridge for IVD raw materials, offering validated antibodies, bulk ancillary reagents, and rare target raw-material procurement. For researchers focused on mitochondrial biology and autophagy, we provide reliable polyclonal antibodies like this anti-FUNDC1 antibody to support your assay development and biomarker discovery workflows. Contact us to explore customized sourcing solutions tailored to your pipeline.