Anti-Fukutin Monoclonal Antibody for WB, IHC-P, ELISA - O75072

Core Product Specifications and Parameters

Biological Background: Fukutin Function and Localization

• FKTN encodes ribitol-5-phosphate transferase FKTN (Fukutin), a transmembrane protein that catalyzes the transfer of a ribitol-phosphate from CDP-ribitol to the phosphorylated O-mannosyl trisaccharide on alpha-dystroglycan (DAG1), initiating the ribitol 5-phosphate tandem repeat essential for ligand binding. Related references: PMID:26923585 PMID:27194101 PMID:29477842 PMID:17034757 PMID:25279699
• FKTN is required for normal localization of POMGNT1 in Golgi membranes and for normal POMGNT1 activity, and may interact with a large complex reinforcing muscle membrane integrity. Related references: PMID:17034757 PMID:25279699
• Localizes to the Golgi apparatus membrane, with additional presence in the cytoplasm and nucleus, consistent with its role in glycosylation and potential nuclear functions.
• Widely expressed with highest levels in brain, heart, pancreas, and skeletal muscle; expressed in neurons and retina, but not in glial cells, suggesting a role in neuronal development and function. Related references: PMID:29416295 PMID:11115853
• Mutations in FKTN cause a spectrum of dystroglycanopathies including Fukuyama-type congenital muscular dystrophy (FCMD), limb-girdle muscular dystrophy, cardiomyopathy, and lissencephaly, highlighting its critical role in muscle and brain development.
• Belongs to the fukutin family and is characterized by a signal-anchor and a transmembrane helix, important for its membrane insertion.
• The protein weight is approximately 54 kDa (UniProt: 53724 Da), consistent with the observed band in Western blot applications.

Experimental Guidance and Technical Tips

• For Western blot (WB), consider loading protein from human, mouse, or rat tissue lysates (brain, skeletal muscle, heart) and use a molecular weight marker to confirm the band at ~54 kDa.
• When performing immunohistochemistry on paraffin-embedded sections (IHC-P), antigen retrieval and appropriate positive controls (e.g., human brain or muscle tissue) are recommended; negative controls should include glial-rich regions.
• In ELISA, the antibody may be used for detection of recombinant or native FKTN; pre-coat the antigen under optimized conditions and validate with known positive/negative samples.
• Given the cross-reactivity with mouse and rat, verify reactivity in the intended model system by including appropriate positive and negative controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with one-stop access to validated IVD raw materials, including monoclonal antibodies like this anti-Fukutin antibody for dystroglycanopathy and neurological disorder research. Beyond individual reagents, we supply optimized antibody pairs, bulk ancillary reagents, and can source rare-target raw materials to streamline your assay development. Our expertise supports every stage from concept to clinic, ensuring reliable procurement for your diagnostic pipeline.