Anti-FUBP3 Polyclonal Antibody for WB, ELISA - Q96I24

Core Product Specifications and Parameters

Biological Background: FUBP3 Function and Localization

• FUBP3 (also known as FBP3) is a far upstream element-binding protein 3 that may interact with single-stranded DNA from the far-upstream element (FUSE) and potentially activates gene expression.
• The protein is localized to the nucleus, consistent with its role in transcriptional regulation.
• Post-translational modifications include acetylation, phosphorylation, isopeptide bonds, and ubiquitin-like conjugations, which may modulate its activity.
• Alternative splicing of the FUBP3 gene yields multiple transcript variants, contributing to functional diversity.
• FUBP3 is expressed in various cell lines, indicating a broad regulatory role.
• It functions as a DNA-binding protein, as evidenced by its association with FUSE elements and involvement in transcription.
• Proteomic identification confirms its presence in nuclear fractions, supporting its role in gene regulation.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 65-260 of human FUBP3, a central region likely conserved between human and mouse; consider testing cross-reactivity in both species.
• For Western blot, the predicted molecular weight is approximately 62 kDa; select gel percentage and transfer conditions suitable for this size range.
• ELISA protocols may be used to quantify FUBP3 levels in cell lysates; validate with appropriate standards.
• As a nuclear protein, consider nuclear extraction methods to enrich the target in sample preparation.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your dedicated partner for sourcing high-quality IVD raw materials, offering validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents. Our expertise in transcription regulation research supports your development needs for FUBP3 detection tools, ensuring a seamless supply chain from concept to clinic.