Anti-FUBP1 Rabbit Monoclonal Antibody for IP, IF/ICC, IF-P, IHC-P, ELISA - Q96AE4

Core Product Specifications and Parameters

Biological Background: FUBP1 Function and Localization

• FUBP1 (Far upstream element-binding protein 1), also known as FBP, FUBP, or DNA helicase V (hDH V), is encoded by the FUBP1 gene.
• It functions as a transcriptional regulator by binding to the single-stranded far-upstream element (FUSE) upstream of the MYC promoter, potentially acting as both an activator and repressor of MYC transcription.
• Subcellular localization is primarily nuclear, consistent with its role in transcription regulation.
• Post-translational modifications include acetylation, methylation, phosphorylation, and ubiquitination, as indicated by UniProt keywords.
• Alternative splicing produces multiple isoforms, expanding its functional diversity.
• It belongs to a class of DNA-binding proteins involved in transcription regulation and is part of the reference proteome.
• No tissue-specific expression data is available from this entry, but it has been identified through direct protein sequencing and proteomics.
• The calculated molecular weight is approximately 67.6 kDa, but migration at 68 kDa is often observed.

Experimental Guidance and Technical Tips

• The immunogen sequence (aa 545-644) near the C-terminus may confer specific detection of full-length FUBP1; consider validating in the relevant sample system.
• For immunoprecipitation (IP), optimize antibody concentration and use appropriate lysis buffers to maintain protein complexes.
• For immunofluorescence (IF/ICC and IF-P), confirm nuclear localization by counterstaining with a DNA dye.
• For immunohistochemistry on paraffin sections (IHC-P), antigen retrieval conditions may need optimization for formalin-fixed tissues.
• For ELISA applications, evaluate the antibody as either a capture or detection reagent in sandwich assay formats.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and researchers with a reliable supply of IVD raw materials, including this FUBP1 rabbit monoclonal antibody for transcription regulation and cancer research. Our one-stop platform offers validated monoclonal and polyclonal antibodies, bulk ancillary reagents, and tailored sourcing for rare targets, helping to streamline your development from concept to clinic.