Anti-FUBP1 Polyclonal Antibody for WB, IHC-P, IF/ICC, IP, ELISA - Q96AE4

Core Product Specifications and Parameters

Biological Background: FUBP1 Function and Localization

• FUBP1 (Far upstream element-binding protein 1), also known as DNA helicase V (hDH V), is a single-stranded DNA-binding protein that functions as a transcriptional regulator.
• It specifically binds to the far-upstream element (FUSE) upstream of the MYC promoter, thereby modulating MYC gene expression; it can act as both an activator and a repressor of transcription.
• The protein is predominantly localized in the nucleus, consistent with its role in transcription regulation.
• FUBP1 undergoes multiple post-translational modifications, including phosphorylation, acetylation, and methylation, which may fine-tune its activity and interactions.
• Alternative splicing generates multiple transcript variants, contributing to functional diversity; the canonical isoform has a calculated molecular weight of ~67.6 kDa.
• It is classified under keywords such as DNA-binding, transcription regulation, and Ubl conjugation, and is part of the reference proteome.

Experimental Guidance and Technical Tips

• The immunogen covers a large internal region (aa 302–644) of human FUBP1, which may enhance detection of both endogenous and overexpressed protein across multiple applications.
• For western blot, the expected molecular weight is approximately 68 kDa; always confirm band size in your sample and use appropriate controls.
• This antibody is suitable for IHC-P and IF/ICC; consider antigen retrieval and permeabilization conditions specific to your tissue or cell type, and validate the staining pattern against known nuclear localization.
• When using for immunoprecipitation, include a no-antibody control and confirm pull-down efficiency by analyzing eluates alongside input samples.
• Cross-reactivity with mouse and rat has been listed; verify reactivity in your experimental models, particularly when working with orthologs that may show minor sequence divergence.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a comprehensive portfolio of IVD raw materials, including validated antibodies and recombinant proteins for transcriptional regulation and oncology targets like FUBP1. As a one-stop sourcing partner for diagnostic manufacturers and research laboratories, CamelBio supports your workflow with optimized polyclonal and monoclonal antibodies, antibody pairs, and ancillary reagents. For rare or emerging targets, our sourcing network helps secure high-quality materials to accelerate your assay development from concept to clinic.