Anti-FTSJ3 Rabbit Polyclonal Antibody for WB, ELISA - Q8IY81

Core Product Specifications and Parameters

Biological Background: FTSJ3 Function and Localization

• FTSJ3 (also known as SPB1, EPCS3) is a pre-rRNA 2'-O-ribose RNA methyltransferase that belongs to the ftsJ family of methyltransferases. It plays a critical role in ribosome biogenesis by catalyzing 2'-O-methylation of the 34S pre-rRNA, facilitating its processing to the mature 18S rRNA and subsequent 40S ribosomal subunit formation.
• During HIV-1 infection, FTSJ3 is recruited to the viral RNA genome via interaction with TARBP2/TRBP, where it performs internal 2'-O-methylation, preferentially on adenosine residues. This methylation enables the virus to evade innate immune detection by the sensor IFIH1/MDA5. Related references: PMID:30626973
• The UniProt record annotates FTSJ3 with keywords including Citrullination, Isopeptide bond, Phosphoprotein, and Ubl conjugation, indicating that post-translational modifications may regulate its activity or interactions.
• FTSJ3 localizes predominantly to the nucleolus, consistent with its role in early ribosomal RNA processing and modification.
• FTSJ3 is an S-adenosyl-L-methionine (SAM)-dependent methyltransferase, using SAM as a methyl donor for its RNA modification activity.
• As a Reference proteome member, FTSJ3 is conserved across species and has a calculated molecular weight of approximately 96.6 kDa (UniProt), though it runs at ~97 kDa in SDS-PAGE, likely due to modifications.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the C‑terminal region (aa 608–847) of human FTSJ3, which may provide specificity for full‑length and relevant isoforms. Consider verifying detection of the expected 97 kDa band in Western blotting using appropriate cell or tissue lysates.
• For ELISA, the polyclonal nature of this antibody may afford robust signal detection; however, we recommend validating against the immunogen peptide or recombinant protein as a positive control.
• Given FTSJ3’s nucleolar localization, nuclear extracts may enrich the target and enhance signal in both WB and ELISA applications.
• As FTSJ3 is expressed across many tissues, a wide variety of human cell lines (e.g., HeLa, HEK293) can serve as positive control lysates.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio bridges global diagnostic manufacturers and research institutions with a comprehensive portfolio of IVD raw materials and technical services. This anti‑FTSJ3 antibody is part of our expanding catalog targeting nucleolar proteins and host‑virus interaction factors, supporting assay development in infectious disease and ribosome research. Beyond individual antibodies, we offer validated antibody pairs, bulk ancillary reagents, and customized sourcing solutions to accelerate your project from concept to clinic.