Anti-FTO Rabbit Polyclonal Antibody for WB, IP, ELISA - Q9C0B1

Core Product Specifications and Parameters

Biological Background: FTO Function and Localization

• FTO (fat mass and obesity-associated protein) is an alpha-ketoglutarate-dependent dioxygenase that functions as an RNA demethylase, catalyzing the oxidative demethylation of N6-methyladenosine (m6A) in mRNA, U6 snRNA, and the 5' cap m6A(m) modification. Related references: PMID:22002720, PMID:25452335, PMID:26457839, PMID:30197295, PMID:28002401
• It also demethylates N1-methyladenine in tRNAs and can repair alkylated DNA/RNA in vitro, though the in vivo relevance is uncertain. Related references: PMID:30197295, PMID:18775698, PMID:20376003
• By modulating m6A levels, FTO regulates mRNA stability, splicing, and translation, thereby influencing adipogenesis, body weight, and energy homeostasis. Related references: PMID:18775698, PMID:26287746
• FTO is ubiquitously expressed with highest levels in the adrenal glands and brain (particularly hypothalamus and pituitary), and is overexpressed in certain acute myeloid leukemias (AML) with specific genetic rearrangements. Related references: PMID:17434869, PMID:17496892, PMID:28017614
• Subcellular localization includes the nucleus (with enrichment in nuclear speckles) and the cytoplasm, reflecting its roles in RNA processing and translation.
• FTO belongs to the ALKBH family of dioxygenases and contains an iron- and 2-oxoglutarate-binding domain essential for its catalytic activity.
• Post-translational modifications include acetylation, phosphorylation, and ubiquitin-like conjugation, which may regulate its stability and activity.
• FTO acts as an oncogene in AML by demethylating and stabilizing mRNAs of leukemogenic factors such as MYC and CEBPA. Related references: PMID:28017614, PMID:29249359

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 327–504 (C-terminal region) of human FTO. This polyclonal antibody is expected to recognize multiple epitopes, providing robust detection in denaturing and native conditions.
• For Western blot (WB), the predicted molecular weight is ~58 kDa; validate expression in positive control lysates (e.g., brain, AML cell lines) and consider using phosphatase inhibitors to preserve phosphorylation states.
• In immunoprecipitation (IP), the antibody may capture FTO–RNA complexes; crosslink or use RNase-free conditions if RNA–protein interactions are to be preserved.
• For ELISA, confirm specificity via recombinant FTO coating and competitive inhibition assays; the listed cross-reactivity with mouse and rat allows multi-species study designs.
• When comparing expression across tissues or treatments, confirm equal loading and account for FTO’s dynamic regulation by metabolic and stress pathways.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides comprehensive access to high-quality IVD raw materials, including validated antibodies, antigen pairs, and ancillary reagents for epitranscriptomics and metabolic disease research. Our FTO polyclonal antibody supports your diagnostic and research needs in obesity, adipogenesis, and leukemia studies. Explore our sourcing solutions for rare target antibodies and bulk supplies.