Anti-FTO Rabbit Monoclonal Antibody for WB, IHC-P, IF/ICC, IP, ELISA - Q9C0B1

Core Product Specifications and Parameters

Biological Background: FTO Function and Localization

• FTO (Fat mass and obesity-associated protein) is an alpha-ketoglutarate-dependent dioxygenase that functions as an RNA demethylase. Also known as ALKBH9, it belongs to the AlkB family of dioxygenases.
• It mediates oxidative demethylation of N6-methyladenosine (m6A) in mRNA, the most prevalent internal modification in higher eukaryotes, thereby regulating mRNA expression and stability. Related references: PMID:22002720, PMID:25452335, PMID:26457839, PMID:30197295
• FTO also demethylates N6,2'-O-dimethyladenosine (m6Am) in mRNA caps, affecting mRNA stability by promoting decapping, and acts on U6 snRNA m6A. Related references: PMID:28002401, PMID:30197295
• It functions as a tRNA demethylase by removing N1-methyladenine and has low activity toward alkylated DNA/RNA repair, though its in vivo role in DNA repair is uncertain. Related references: PMID:30197295, PMID:18775698, PMID:20376003
• Subcellular localization is primarily nuclear, with presence in nuclear speckles and cytoplasm.
• FTO is ubiquitously expressed, with high expression in adrenal glands, brain (hypothalamus and pituitary), and is overexpressed in certain acute myeloid leukemias with specific genetic alterations. Related references: PMID:17434869, PMID:17496892, PMID:28017614
• Post-translational modifications include acetylation, phosphorylation, and ubiquitin-like conjugation, which may regulate its activity and stability.
• It plays a crucial role in regulating fat mass, adipogenesis, body weight, and energy homeostasis, and is involved in thermogenesis and adipocyte differentiation. Related references: PMID:18775698, PMID:20376003, PMID:26287746
• Oncogenic role in acute myeloid leukemia by demethylating m6A on oncogene transcripts like MYC, CEBPA, ASB2, and RARA, promoting their expression. Related references: PMID:28017614, PMID:29249359

Experimental Guidance and Technical Tips

• The immunogen is a peptide from the N-terminal region (aa 1-100), which may detect full-length FTO and possibly some isoforms. Include positive and negative controls (e.g., FTO-overexpressing lysates or siRNA-treated samples) to confirm specificity.
• For Western blot (WB), run reduced and denatured protein samples; the expected band size is ~58 kDa. Use appropriate loading controls and blocking conditions.
• In IHC-P and IF/ICC, test different fixation and antigen retrieval methods. Paraffin-embedded sections may require heat-induced epitope retrieval. Validate staining using known positive tissues (brain, adrenal) and include isotype controls.
• For immunoprecipitation (IP), the antibody may be suitable for capturing native FTO complexes. Optimize lysis buffer conditions and antibody amount per given total protein.
• For ELISA, the antibody can be utilized in both direct and sandwich formats; optimize coating and detection conditions according to your specific assay requirements.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with one-stop access to IVD raw materials. This anti-FTO antibody supports research into obesity, metabolism, and acute myeloid leukemia. As your sourcing bridge, CamelBio offers validated antibody pairs, optimized recombinant proteins, bulk ancillary reagents, and custom raw-material solutions to accelerate your diagnostic development from concept to clinic.