Anti-FTO Rabbit Monoclonal Antibody for WB, IHC-P, ELISA - Q9C0B1

Core Product Specifications and Parameters

Biological Background: FTO Function and Localization

• FTO is an alpha-ketoglutarate-dependent dioxygenase and RNA demethylase that functions as a critical regulator of fat mass, adipogenesis, and energy homeostasis. Related references: PMID:22002720, PMID:25452335, PMID:26457839
• It specifically demethylates N6-methyladenosine (m6A) in mRNA, thereby influencing mRNA expression and stability. Related references: PMID:30197295
• FTO also demethylates N6,2'-O-dimethyladenosine (m6A(m)) in the 5' cap of mRNAs, promoting susceptibility to decapping and affecting transcript stability. Related references: PMID:28002401, PMID:30197295
• Additionally, FTO acts as a tRNA demethylase, removing N1-methyladenine from various tRNAs. Related references: PMID:30197295
• FTO plays a key role in the regulation of body weight and adipogenesis, contributing to body size and fat accumulation. Related references: PMID:18775698, PMID:20376003
• It is involved in the regulation of thermogenesis and the control of adipocyte differentiation into brown or white fat cells. Related references: PMID:26287746
• FTO exerts an oncogenic role in certain acute myeloid leukemias by demethylating m6A on leukemic oncogene transcripts (e.g., MYC, CEBPA), leading to enhanced expression and cell transformation. Related references: PMID:28017614, PMID:29249359
• FTO localizes to the nucleus, nuclear speckles, and cytoplasm.
• FTO is ubiquitously expressed, with particularly high levels in the adrenal glands, brain (especially hypothalamus and pituitary), and in specific acute myeloid leukemia subtypes with defined genetic lesions. Related references: PMID:17434869, PMID:17496892, PMID:28017614

Experimental Guidance and Technical Tips

• The immunogen corresponds to an internal sequence (amino acids 327–504) of human FTO, which may help reduce interference from post-translational modifications or alternative splicing variants.
• For western blotting, use KO‑validated cell lysates as negative controls to confirm band specificity. The expected molecular weight is approximately 58 kDa.
• Optimize antibody dilution and incubation conditions for your specific sample type and detection system.
• For immunohistochemistry, antigen retrieval protocols should be empirically determined for each tissue type. Consider testing both heat-induced and enzymatic retrieval methods.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a one-stop sourcing bridge for IVD assay raw materials, including KO-validated antibodies like this FTO mAb, supporting epitranscriptomic, metabolic, and oncology research. We supply validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and assist in sourcing rare-target raw materials to streamline your diagnostic development pipeline.