Anti-FTCD Polyclonal Antibody for WB and ELISA - O95954

Core Product Specifications and Parameters

Biological Background: FTCD Function and Localization

• FTCD (formimidoyltransferase-cyclodeaminase), also known as LCHC1, is a bifunctional enzyme that channels one-carbon units from formiminoglutamate into the folate pool.
• It sequentially catalyzes the transfer of a formimino group to tetrahydrofolate (transferase activity) and the deamination to form methenyltetrahydrofolate (cyclodeaminase activity), a key step in histidine degradation.
• Beyond its enzymatic role, FTCD binds to and promotes bundling of vimentin intermediate filaments originating from the Golgi apparatus, linking folate metabolism to cytoskeletal organization.
• The protein localizes to the cytoplasm, Golgi apparatus, and centrosome/centriole, indicating dynamic subcellular distribution.
• FTCD undergoes phosphorylation and alternative splicing, producing multiple isoforms that may have distinct functions or localization.
• Mutations in the FTCD gene are associated with glutamate formiminotransferase deficiency, an inborn error of histidine metabolism.
• The protein has a predicted molecular weight of approximately 59 kDa and belongs to the folate-binding protein family.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a C-terminal region (amino acids 412–541) of FTCD, which may allow detection of both full-length and certain truncated isoforms in Western blot.
• For WB, expect a predominant band near 59 kDa in human, mouse, and rat lysates; validate the specificity in your own sample system and consider loading controls.
• Cross-reactivity with mouse and rat suggests conservation of the epitope, but it is advisable to confirm performance in non-human species with appropriate positive controls.
• For ELISA applications, consider using the antibody as a capture or detection reagent after titrating against recombinant FTCD or cell lysates.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive partner for IVD raw material sourcing, supporting diagnostic manufacturers and researchers from concept to clinic. This FTCD polyclonal antibody is part of our broad portfolio for histidine metabolism and vimentin-related studies, and we can supply validated antibody pairs, bulk ancillary reagents, and assist with sourcing rare-target raw materials. Let CamelBio streamline your procurement with reliable, scalable solutions.