Anti-FTCD Monoclonal Antibody for WB, ELISA - O95954

Core Product Specifications and Parameters

Biological Background: FTCD Function and Localization

• FTCD (Formimidoyltransferase-cyclodeaminase, also known as LCHC1) is a multifunctional folate-dependent enzyme that exhibits both transferase and deaminase activities, playing a critical role in histidine metabolism by channeling one-carbon units from formiminoglutamate into the folate pool.
• The gene undergoes alternative splicing, generating multiple isoforms that may possess distinct functional properties.
• FTCD promotes the bundling of vimentin filaments that originate from the Golgi apparatus, linking folate metabolism to cytoskeletal organization.
• Subcellularly, FTCD localizes to the cytoplasm and cytosol, and is also associated with the Golgi apparatus and centrosome/centriole structures, indicating a dynamic intracellular distribution.
• The protein is classified as a lyase and transferase, with key annotated features including folate-binding, phosphoprotein status, and involvement in histidine metabolism.
• Mutations in FTCD are associated with disease variants (as noted in UniProt keywords), suggesting its clinical relevance.

Experimental Guidance and Technical Tips

• The immunogen is a recombinant human FTCD protein; this suggests the antibody may target multiple epitopes, which can enhance detection sensitivity in western blotting.
• For ELISA applications, consider pairing this antibody with a capture antibody to develop a sandwich assay, and validate with recombinant FTCD and native samples.
• When analyzing samples from mouse or rat, confirm cross-reactivity by comparing band patterns or signal intensity against human controls, as sequence homology may influence antibody affinity.
• The observed protein weight of 59 kDa (theoretical ~58.9 kDa) may vary slightly due to post-translational modifications; use appropriate molecular weight markers for accurate sizing.

CamelBio: Your One-Stop Sourcing Bridge

As a dedicated IVD raw-material partner, CamelBio connects diagnostic manufacturers and laboratories with high-quality reagents, including optimized monoclonal antibodies like this anti-FTCD clone for folate metabolism and cytoskeletal research. We facilitate access to validated antibody pairs, bulk ancillary reagents, and customized sourcing for rare targets, streamlining your assay development from concept to clinic.