Anti-FTCD Monoclonal Antibody for WB, ELISA - O95954

Core Product Specifications and Parameters

Biological Background: FTCD Function and Localization

• FTCD (formimidoyltransferase-cyclodeaminase) is a bifunctional enzyme that channels one-carbon units from formiminoglutamate to the folate pool, serving as a central hub in histidine degradation and folate metabolism.
• The enzyme also promotes bundling of vimentin filaments originating from the Golgi, linking one-carbon metabolism with cytoskeletal organization.
• Alternative splicing generates multiple isoforms, which may contribute to functional diversity in different cellular contexts.
• FTCD localizes to the cytoplasm, with enrichment at the Golgi apparatus and centrosome/centriole structures of the microtubule organizing center.
• Post-translational modifications include phosphorylation, as suggested by the Phosphoprotein keyword, potentially regulating its activity or localization.
• Classified as a multifunctional enzyme with distinct transferase and lyase (cyclodeaminase) domains, reflecting its dual catalytic roles.
• Mutations in FTCD are associated with glutamate formiminotransferase deficiency, a disorder of histidine metabolism (Disease variant keyword).

Experimental Guidance and Technical Tips

• The recombinant human FTCD immunogen covers the full-length protein, which may contribute to high specificity; consider validating against endogenous controls in your target species.
• For Western blot, expect a band near 59 kDa; pre-stained markers and appropriate loading controls are recommended to confirm molecular weight.
• When using ELISA, optimize coating antigen and antibody dilution for direct or sandwich formats with recombinant protein or biological samples.
• Cross-reactivity with mouse and rat FTCD has been reported, enabling comparative studies across species; verify sequence homology in your region of interest.
• Include appropriate blocking and washing steps to minimize background, especially if using complex tissue lysates.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers and research laboratories streamlined access to high-quality IVD raw materials, including validated monoclonal antibodies like this anti-FTCD rabbit mAb. Beyond individual reagents, we supply optimized antibody pairs, recombinant proteins, and ancillary bulk reagents to support your histidine metabolism and folate pathway projects. Our sourcing expertise extends to rare or difficult-to-find targets, helping you bridge the gap from concept to clinical application efficiently.