Anti-FSTL5 Polyclonal Antibody for WB, IF-P, ELISA - Q8N475

Core Product Specifications and Parameters

Biological Background: FSTL5 Function and Localization

• FSTL5 (Follistatin-related protein 5), also known as Follistatin-like protein 5, is encoded by the FSTL5 gene in humans.
• It is predicted to be a secreted protein, as indicated by its signal peptide and subcellular localization annotation.
• The protein contains immunoglobulin-like domains and multiple repeat regions, as suggested by its domain architecture annotations.
• FSTL5 is a glycoprotein characterized by several disulfide bonds and calcium-binding properties, which contribute to its structural stability.
• Alternative splicing generates multiple transcript variants, expanding the functional diversity of the protein.
• FSTL5 has been identified through proteomics approaches and is part of the reference proteome.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (aa 21-250), which may target extracellular epitopes; consider this for applications requiring native conformation detection.
• For Western blotting, a band around 96 kDa is expected; validate specificity using positive controls from human, mouse, or rat cell lines/tissues.
• For immunofluorescence, use appropriate permeabilization and blocking steps to detect secreted protein; consider co-staining with organelle markers to confirm localization.
• ELISA applications may benefit from pairing this antibody with another clone recognizing a different epitope; optimize coating and detection conditions.
• Cross-reactivity with mouse and rat allows translational studies, but verify reactivity in your specific experimental system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports your research on follistatin-related proteins and secreted factors with reliable access to validated antibodies, including this polyclonal antibody against FSTL5. As a one-stop sourcing partner for IVD raw materials, we offer optimized monoclonal and polyclonal antibodies, recombinant proteins, and ancillary reagents to streamline your assay development. Whether you require bulk quantities, customized immunogen design, or assistance sourcing rare target reagents, CamelBio bridges your concept to clinic with expert technical consultation and robust supply solutions.