Anti-FSTL4 Polyclonal Antibody for WB, IF/ICC, ELISA - Q6MZW2

Core Product Specifications and Parameters

Biological Background: FSTL4 Function and Localization

• FSTL4 (Follistatin-related protein 4), also called follistatin-like protein 4, is encoded by the FSTL4 gene in humans (GeneID: 23105).
• It is a secreted protein, as indicated by its subcellular localization (UniProt: Secreted).
• The protein contains an immunoglobulin domain, suggesting potential roles in cell-cell recognition or adhesion.
• Keywords indicate calcium and metal binding, likely mediated by conserved domains.
• It is a glycoprotein with disulfide bonds, typical of extracellular proteins.
• Alternative splicing produces multiple isoforms; the canonical isoform has a calculated molecular weight of 93 kDa.
• FSTL4 belongs to the follistatin protein family, which are known to interact with TGF-beta superfamily ligands, though the specific function of FSTL4 remains under investigation.

Experimental Guidance and Technical Tips

• The immunogen includes the N-terminal region (aa 22-250), which may correspond to extracellular domains; ensure the target antigen is accessible in your assay.
• For Western blotting, the predicted molecular weight is ~93 kDa; verify with appropriate controls and consider potential isoforms or glycosylation effects.
• For immunofluorescence/ICC, since FSTL4 is secreted, consider using secretion inhibitors like Brefeldin A to detect intracellular accumulation if staining fixed cells.
• Cross-reactivity with mouse and rat allows for translational studies, but validate species-specific expression patterns.
• For ELISA, pair this polyclonal antibody with a capture antibody or use as a detection reagent after confirming performance.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research labs with reliable access to IVD raw materials like the FSTL4 polyclonal antibody, supporting studies on secreted proteins and signaling pathways. We offer validated antibody pairs, monoclonal and polyclonal antibodies, and bulk ancillary reagents to streamline assay development. For rare or custom targets, our sourcing network helps bridge the gap from concept to clinic.