Anti-FSP1/S100A4 Rabbit Polyclonal Antibody for WB - P26447

Core Product Specifications and Parameters

Biological Background: FSP1/S100A4 Function and Localization

• S100A4 is a member of the S100 family of calcium-binding proteins, also known as calvasculin, metastasin, and FSP1.
• Involved in multiple cellular processes such as motility, angiogenesis, cell differentiation, apoptosis, and autophagy. Related references: PMID:16707441, PMID:23752197, PMID:30713770
• Promotes cell motility and invasiveness by interacting with non-muscle myosin heavy chain IIA (MYH9) to increase soluble myosin-IIA and facilitate chemotaxis. Related references: PMID:16707441
• Modulates the pro-apoptotic function of TP53 by binding to its C-terminal transactivation domain within the nucleus, reducing TP53 protein levels. Related references: PMID:23752197
• Stimulates T-lymphocyte chemotaxis by acting as a chemoattractant complex with PGLYRP1 via CCR5 and CXCR3 receptors. Related references: PMID:26654597, PMID:30713770
• Localizes to the nucleus, cytoplasm, and is secreted into the extracellular space.
• Ubiquitously expressed across human tissues.
• Subject to acetylation, which may modulate its functional activities.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (amino acids 1-101) of human S100A4, which may confer target specificity; validate in your relevant sample system.
• For western blot (WB), the predicted molecular weight is 12 kDa; ensure proper gel percentage and transfer conditions for low-molecular-weight proteins.
• In immunohistochemistry (IHC-P) and immunofluorescence (IF/ICC), consider the nuclear and cytoplasmic localization of S100A4 when evaluating staining patterns.
• For ELISA applications, the antibody may be used for detection of S100A4 in cell lysates or biological fluids; include appropriate positive and negative controls.
• Due to cross-reactivity with mouse and rat, this antibody is suitable for studies using rodent models; confirm expression levels in the tissue of interest.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. For research targeting S100A4 in cancer metastasis and cell motility, we supply this validated rabbit polyclonal antibody along with antibody pairs, bulk ancillary reagents, and custom sourcing for rare targets. Our streamlined procurement ensures consistent quality to accelerate your assay development and manufacturing workflows.