Anti-FSHB Polyclonal Antibody for WB, ELISA - P01225

Core Product Specifications and Parameters

Biological Background: Follitropin Subunit Beta (FSHB) Function and Localization

• FSHB encodes the beta subunit of follicle-stimulating hormone (FSH), which heterodimerizes with the alpha chain CGA to form biologically active follitropin.
• The FSH heterodimer binds to FSHR, a G protein-coupled receptor, on target cells in the ovaries and testes, activating downstream signaling pathways. Related references: PMID:24692546 PMID:2494176
• Follitropin is essential for follicular development in females and spermatogenesis in males. Related references: PMID:407105 PMID:8220432
• The protein is secreted from gonadotroph cells of the anterior pituitary gland.
• It contains multiple disulfide bonds and glycosylation sites, which are critical for structural stability and biological activity.
• Mutations in FSHB are associated with hypogonadotropic hypogonadism, a condition characterized by impaired gonadal function.
• FSHB is a key diagnostic marker and therapeutic target in reproductive medicine and fertility treatments.

Experimental Guidance and Technical Tips

• The immunogen is a recombinant fusion protein spanning amino acids 19-129 of human FSHB. Ensure that the antibody recognizes the native protein in your sample matrix.
• For western blotting, consider using a reducing agent and denaturing conditions; the predicted molecular weight is ~15 kDa, but glycosylation may shift migration.
• For ELISA, this polyclonal antibody can be used as a capture or detection reagent after optimization of coating and blocking conditions.
• Validate the antibody in mouse and rat samples, as cross-reactivity with these species has been reported.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a comprehensive range of IVD raw materials, including validated antibody pairs, recombinant proteins, and ancillary reagents, to streamline your diagnostic assay development. Our portfolio in reproductive health targets such as FSHB supports immunoassay development for fertility testing and endocrine disorders. Partner with us to access reliable, high-quality raw materials and technical services from concept to clinic.