Anti-FRZB Rabbit Polyclonal Antibody for WB, ELISA - Q92765

Core Product Specifications and Parameters

Biological Background: FRZB Function and Localization

• FRZB, also known as Secreted frizzled-related protein 3 (SFRP3), Frezzled, or Fritz, is a member of the soluble frizzled-related protein (sFRP) family.
• sFRPs function as extracellular modulators of Wnt signaling by directly binding to Wnt ligands, thereby regulating cell growth and differentiation.
• FRZB specifically acts as an antagonist of Wnt8 signaling and is critically involved in limb skeletogenesis, regulating chondrocyte maturation and long bone development.
• The protein is secreted and contains a cysteine-rich domain (CRD) and a netrin-like domain, with disulfide bonds contributing to its structure; it is also a glycoprotein.
• Tissue expression is prominent in the cartilaginous cores of long bones during embryonic and fetal development, as well as in the appendicular skeleton, placenta, heart, brain, skeletal muscle, kidney, and pancreas.
• FRZB is implicated in developmental processes and has been associated with disease variants; it is identified as a reference proteome component with potential roles in differentiation.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 166-325 within the netrin-like domain of human FRZB, suggesting the antibody may recognize epitopes in this region.
• For Western blotting, a band around 36 kDa is expected; consider potential post-translational modifications such as glycosylation that may affect migration.
• The antibody cross-reacts with rat and mouse FRZB, enabling multispecies studies; however, validate in the relevant sample system.
• In ELISA applications, the polyclonal nature provides sensitivity but assess specificity using appropriate controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a one-stop IVD raw-material sourcing partner, offering validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents. We support diagnostic manufacturers and researchers with FRZB-targeted tools for studying Wnt signaling and skeletal development, with access to rare-target raw materials and technical consulting from concept to clinic.