Anti-FRS2 Rabbit Monoclonal Antibody for WB, IHC-P, IF/ICC, ELISA - Q8WU20

Core Product Specifications and Parameters

Biological Background: FRS2 Function and Localization

• FRS2 (Fibroblast growth factor receptor substrate 2), also known as SNT-1, is an adapter protein that links activated FGF and NGF receptors to downstream signaling pathways.
• It plays a critical role in the activation of MAP kinases and the phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase, upon ligand-mediated activation of FGFR1.
• FRS2 modulates signaling via SHC1 by competing for a common binding site on NTRK1.
• It is localized to the endomembrane system.
• Highly expressed in heart, brain, spleen, lung, liver, skeletal muscle, kidney, and testis.
• Classified as a lipoprotein and membrane-associated phosphoprotein, with a myristate modification and involvement in ubiquitin conjugation (Ubl conjugation).

Experimental Guidance and Technical Tips

• For Western blot, consider using whole cell lysates from FRS2-expressing tissues like heart, brain, or liver, and validate the band at approximately 57 kDa.
• In IHC-P, optimize antigen retrieval and antibody dilution; FRS2 is expressed in multiple organs, so include appropriate tissue controls.
• For IF/ICC, use fixation and permeabilization methods suited for endomembrane-localized proteins; co-stain with organelle markers if needed.
• For ELISA, use recombinant FRS2 protein as a standard and titrate the antibody to determine optimal coating and detection concentrations.
• Since the antibody cross-reacts with mouse and rat, verify species-specific reactivity with positive and negative controls in your experimental system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers, labs, and research institutes a one-stop access to IVD raw materials and services, covering every stage from concept to clinic. For FGFR signaling research and associated assay development, we supply validated antibody pairs, optimized monoclonal and polyclonal antibodies such as this FRS2 Rabbit mAb, along with bulk ancillary reagents and rare target raw-material sourcing to help accelerate your project timelines.