Anti-FREM2 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - Q5SZK8

Core Product Specifications and Parameters

Biological Background: FREM2 Function and Localization

• FREM2 is a large multidomain extracellular matrix protein, also referred to as ECM3 homolog, belonging to the FRAS1 family.
• It is essential for the maintenance of skin and renal epithelial integrity and epidermal adhesion.
  Related references: PMID:15838507
• FREM2 is involved in eyelid and anterior eye segment development.
  Related references: PMID:29688405, PMID:30802441
• The protein localizes at the cell membrane and contains transmembrane domains, a signal peptide, and calcium-binding motifs.
• It undergoes alternative splicing and glycosylation; mutations in FREM2 are linked to developmental disorders such as Fraser syndrome.
• Keywords: Alternative splicing, Calcium, Cell adhesion, Cell membrane, Developmental protein, Disease variant, Glycoprotein, Membrane, Metal-binding, Reference proteome, Repeat, Signal, Transmembrane.

Experimental Guidance and Technical Tips

• The immunogen spans the extracellular region (aa 47-300), which may enhance recognition of native and denatured FREM2 in WB and IF/ICC applications.
• For WB, consider using lysates from human skin or kidney cell lines given the protein's expression profile.
• For IF/ICC, permeabilization may be required to access the intracellular epitope; validate with appropriate controls.
• ELISA can be used for quantitative detection; optimize coating buffer and antibody concentration for best signal-to-noise ratio.
• Cross-reactivity with mouse is reported; verify in the relevant experimental system.

CamelBio: Your One-Stop Sourcing Bridge

As a premier IVD raw-material partner, CamelBio provides diagnostic manufacturers and researchers with a one-stop supply chain for high-quality antibodies, including validated polyclonal antibodies like anti-FREM2, alongside monoclonal antibodies and antibody pairs. Our portfolio supports customer projects in epithelial biology and genetic disease research, with reliable access to bulk ancillary reagents and sourcing for rare targets such as FRAS1-family extracellular matrix proteins.