Anti-FRA1 Monoclonal Antibody for WB, ELISA - P15407

Core Product Specifications and Parameters

Biological Background: FRA1 Function and Localization

• Fos-related antigen 1 (FRA1) is encoded by the FOSL1 gene (also known as FRA1) and belongs to the Fos family of transcription factors.
• FRA1 localizes predominantly to the nucleus, consistent with its role as a transcription factor.
• It is a DNA-binding protein, indicating direct interaction with genomic regulatory regions to modulate gene expression.
• The FRA1 protein undergoes alternative splicing, producing multiple isoforms that may have distinct functional properties.
• Post-translational modifications include phosphorylation, which can regulate its activity, stability, and protein interactions.
• FRA1 has been identified in high-throughput proteomics studies, validating its endogenous expression in human cells (Reference proteome).

Experimental Guidance and Technical Tips

• The antibody’s immunogen corresponds to the N-terminal region (amino acids 1–100) of human FRA1. When designing similar immunogens, consider targeting conserved regions for broader reactivity or unique regions for isoform specificity.
• For Western blot, the predicted molecular weight is approximately 29 kDa; verify the band size in your sample lysates, keeping in mind potential shifts due to phosphorylation or other modifications.
• In ELISA, the antibody can be used as a capture or detection reagent; optimize coating concentrations and validate in relevant human sample matrices.
• Because FRA1 is a nuclear protein, complete cell lysis and nuclear extraction protocols are recommended for robust detection.
• Include positive and negative controls: known FRA1-expressing cell lines (e.g., certain cancer cell lines) and knockdown/knockout samples to confirm signal specificity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop sourcing bridge for IVD raw materials, offering validated antibody pairs, monoclonal antibodies, and ancillary reagents for transcription factor targets like FRA1. We support diagnostic manufacturers and researchers worldwide with reliable supply chains and technical expertise, from concept to clinic.