Anti-FPR2 Rabbit Polyclonal Antibody for IF/ICC and ELISA - P25090

Core Product Specifications and Parameters

Biological Background: FPR2 Function and Localization

• FPR2 (alias ALX, FPRL1, LXA4R, HM63) is a pattern recognition GPCR that senses formylated peptides from pathogens and damaged mitochondria. Related references: PMID:1374236 PMID:25605714 PMID:35217703
• Ligand binding activates G(i)/GNAI1, inhibiting adenylate cyclase and lowering intracellular cAMP levels. Related references: PMID:40080544
• Recognizes chemically diverse endogenous ligands: chemokine FAM19A5 (macrophage chemotaxis), long‑chain ceramides (inhibit thermogenesis), humanin and amyloid‑β (neuroprotection). Related references: PMID:40080544 PMID:11689470 PMID:35365641
• Oligomerization status tunes downstream signaling: ANXA1‑bound FPR2 homodimers promote anti‑inflammatory p38 MAPK pathway, while FPR1:FPR2 heterodimers trigger pro‑apoptotic JNK signaling.
• The receptor resides at the cell membrane and contains seven transmembrane helices.
• Tissue expression includes lung, bone marrow, neutrophils, spleen, and testis, consistent with innate immune function.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N‑terminal 1‑100 amino acids of human FPR2; the antibody likely recognizes an extracellular epitope, which may be advantageous for live‑cell staining in IF/ICC after proper fixation.
• For IF/ICC, consider using permeabilization and fixation conditions suited for membrane proteins. For ELISA, coating with recombinant FPR2 protein or synthetic peptide may be suitable; validate in the relevant sample system.
• The antibody cross‑reacts with mouse and rat, allowing its use in translational models; however, individual validation in those species is advised.

CamelBio: Your One‑Stop Sourcing Bridge

CamelBio connects diagnostic manufacturers and research labs with a comprehensive portfolio of IVD raw materials. This anti‑FPR2 polyclonal antibody, with its validated applications in immunofluorescence and ELISA, supports inflammation and innate immunity research. Beyond single‑antibody procurement, CamelBio offers one‑stop access to antibody pairs, bulk ancillary reagents, and tailored sourcing services to accelerate your assay development—from early feasibility to clinical realization.