Anti-FPGS Monoclonal Antibody for WB, ELISA - Q05932

Core Product Specifications and Parameters

Biological Background: FPGS Function and Localization

• FPGS (Folylpolyglutamate synthase) is a mitochondrial enzyme that catalyzes the ATP-dependent addition of glutamate residues to folate and folate derivatives, converting them to polyglutamate forms.
• This polyglutamation enables cellular retention of folates, as polyglutamates are poorly transported across membranes, and increases their affinity for folate-dependent enzymes.
• FPGS is central to one-carbon metabolism, providing essential substrates for purine, pyrimidine, and amino acid biosynthesis.
• The enzyme also metabolizes the antifolate drug methotrexate (MTX) to polyglutamate derivatives, influencing intracellular drug retention and pharmacological activity.
• Alternative initiation and splicing generate multiple isoforms; the primary isoform contains a mitochondrial transit peptide and localizes to the mitochondrial inner membrane and matrix, while cytoplasmic localization has also been observed.
• FPGS requires magnesium as a cofactor and ATP for catalysis; post-translational modifications include acetylation and phosphorylation, which may regulate its activity.

Experimental Guidance and Technical Tips

• The immunogen covers the C-terminal region (aa 450–587) of human FPGS, which may help detect specific isoforms or avoid epitope masking in the catalytic domain.
• In WB, the full-length mitochondrial precursor is predicted at ~65 kDa; validate the observed band in your cell or tissue lysate system.
• Cross-reactivity is confirmed for human and rat; for other species, check sequence homology of the immunogen region before use.
• For ELISA, consider pairing with a capture antibody or using recombinant FPGS standard to optimize assay sensitivity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio empowers diagnostic manufacturers, labs, and research institutes with a streamlined, one-stop supply chain for IVD raw materials—from antibody discovery to commercial kit production. For projects focused on folate metabolism, methotrexate monitoring, or mitochondrial enzymology, we offer validated FPGS antibody pairs, optimized monoclonal antibodies, and bulk ancillary reagents to accelerate your assay development. Beyond this product, CamelBio’s integrated platform supports rare-target sourcing, custom antibody generation, and technical consulting, ensuring you have the right materials at every stage from concept to clinic.