Anti-FOXP3 Rabbit Polyclonal Antibody for WB and ELISA - Q9BZS1

Core Product Specifications and Parameters

Biological Background: FOXP3 Function and Localization

• FOXP3 (Forkhead box protein P3), also known as scurfin, is a transcriptional regulator crucial for the development and inhibitory function of regulatory T-cells (Treg). (PubMed:17377532, PubMed:21458306, PubMed:23947341) Related references: PMID:17377532 PMID:21458306 PMID:23947341
• It plays an essential role in immune system homeostasis by maintaining Treg lineage stability and modulating conventional T-cell expansion and function. (PubMed:23169781) Related references: PMID:23169781
• FOXP3 can function as both a transcriptional repressor and activator, depending on interactions with other transcription factors, histone acetylases, and deacetylases. (PubMed:17377532, PubMed:21458306, PubMed:23947341) Related references: PMID:17377532 PMID:21458306 PMID:23947341
• The suppressive activity of Tregs involves FOXP3-mediated activation of genes like CTLA4 and TNFRSF18, alongside repression of cytokines IL2 and IFNG. (PubMed:17377532, PubMed:21458306, PubMed:23947341) Related references: PMID:17377532 PMID:21458306 PMID:23947341
• FOXP3 inhibits cytokine production and T-cell effector function by repressing RELA and NFATC2 transcription factors. (PubMed:15790681) Related references: PMID:15790681
• It represses IL2 transcription via association with histone acetylase KAT5 and histone deacetylase HDAC7. (PubMed:17360565) Related references: PMID:17360565
• FOXP3 can activate or repress genes by interacting with RUNX1, and also inhibits Th17 differentiation by antagonizing RORC and RORA. (PubMed:17377532, PubMed:18368049, PubMed:18354202) Related references: PMID:17377532 PMID:18368049 PMID:18354202
• It is localized in the nucleus and cytoplasm and undergoes post-translational modifications including acetylation, phosphorylation, and ubiquitination; the protein contains a zinc-finger domain and is associated with diseases like IPEX (immune dysregulation, polyendocrinopathy, enteropathy, X-linked syndrome).

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (aa 70–180), which may be suitable for detecting both full-length and some alternative isoforms.
• For Western blot, consider using whole-cell lysates from human or rat Treg cells; a band around 47 kDa is expected.
• In ELISA, validate using recombinant FOXP3 protein or cell lysates from FOXP3-expressing cells.
• Polyclonal nature may provide robust detection but consider lot-to-lot consistency testing.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines IVD raw-material procurement from concept to clinic, offering validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target sourcing. This anti-FOXXP3 antibody supports immunology and autoimmune disease research, backed by our integrated technical services and consulting. Reach out to explore how we can accelerate your diagnostic development with reliable, one-stop supply solutions.