Anti-FOXP3 Monoclonal Antibody for WB, IP, ELISA - Q9BZS1

Core Product Specifications and Parameters

Biological Background: FOXP3 Function and Localization

• Forkhead box protein P3 (FOXP3), also known as Scurfin, is a transcription factor essential for immune regulation.
• Crucial for the development and inhibitory function of regulatory T-cells (Treg) and for maintaining immune homeostasis by ensuring Treg suppressive function and stability, and modulating conventional T-cell expansion and function. Related references: PMID:17377532 PMID:21458306 PMID:23947341 PMID:23169781
• Functions as a transcriptional repressor or activator, depending on interactions with cofactors such as histone acetylases and deacetylases. Related references: PMID:17377532 PMID:21458306 PMID:23947341
• Coordinates expression of immune-related genes: activates CTLA4 and TNFRSF18 while repressing IL2 and IFNG. Related references: PMID:17377532 PMID:21458306 PMID:23947341
• Suppresses cytokine production by inhibiting RELA and NFATC2, and mediates IL2 repression via interactions with KAT5 and HDAC7. Related references: PMID:15790681 PMID:17360565
• Interacts with RUNX1 to regulate target genes, and with IKZF4 to repress IL2 and IFNG. Related references: PMID:17377532
• Inhibits Th17 differentiation by antagonizing RORC, favoring Treg development. Related references: PMID:18368049
• Subcellular localization: Predominantly nuclear, also found in cytoplasm. Subject to post-translational modifications including acetylation and phosphorylation, which modulate its activity.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the C-terminal region (aa 286-431) of human FOXP3, which includes the forkhead DNA-binding domain. This region is highly conserved; however, cross-reactivity data is currently limited to human samples.
• For western blotting, the predicted molecular weight of FOXP3 is approximately 47 kDa. A nuclear-rich lysate from Treg cells is recommended for clear detection.
• For immunoprecipitation, consider using nuclear extraction protocols to enrich for transcription factors and validate the antibody’s binding capacity in the relevant cellular context.
• In ELISA, using immobilized recombinant FOXP3 protein or lysates from FOXP3-expressing cells can help assess binding. Titration of the detection antibody and appropriate blocking are advised.
• Given the predominantly nuclear localization, nuclear fractionation may enhance signal in both WB and IP applications.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio connects diagnostic manufacturers, labs, and research institutes with a comprehensive portfolio of IVD raw materials, technical services, and consulting—from concept to clinic. This FOXP3 Rabbit mAb supports immunology research and Treg-related assay development, reflecting our commitment to supplying validated antibody pairs, bulk ancillary reagents, and rare-target raw materials. Whatever your sourcing needs, including optimized monoclonal/polyclonal antibodies for diagnostic pipelines, CamelBio streamlines procurement to accelerate your project timeline.