Anti-FOXP1 Monoclonal Antibody for WB, IHC-P, IF/ICC, ELISA - Q9H334

Core Product Specifications and Parameters

Biological Background: FOXP1 Function and Localization

• FOXP1, a member of the forkhead box family, functions primarily as a transcriptional repressor. It directly represses the CSF1R promoter via NCOR2 corepressor and modulates monocyte/macrophage differentiation (PubMed:15286807, PubMed:18347093, PubMed:18799727). Related references: PMID:15286807 PMID:18347093 PMID:18799727
• In B cells, FOXP1 cooperates with NF-κB signaling to inhibit caspase-dependent apoptosis, promoting cell expansion. It also negatively regulates T follicular helper cell differentiation (PubMed:25267198). Related references: PMID:25267198
• FOXP1 is crucial for lung epithelium development, restricting goblet cell fate in cooperation with FOXP4, and for cardiac muscle cell proliferation.
• It directs spinal motor neuron columnar organization, contributing to LMC and PGC formation and axon guidance, working with Hox proteins.
• Localized in the nucleus, FOXP1 undergoes phosphorylation, ubiquitination, and binds DNA via its zinc-finger forkhead domain. It is implicated in various diseases and chromosomal rearrangements.
• In angiogenesis, FOXP1 suppresses SEMA5B to regulate endothelial cell behavior (PubMed:24023716). It also negatively regulates androgen receptor signaling (PubMed:18640093) and activates FBXL7 transcription under AURKA control (PubMed:28218735). Related references: PMID:24023716 PMID:18640093 PMID:28218735
• The ESC-specific isoform 8 maintains pluripotency by stimulating pluripotency genes and binding distinct DNA motifs (PubMed:21924763). Related references: PMID:21924763

Experimental Guidance and Technical Tips

• For Western blot (WB), the antibody detects a band at approximately 75 kDa. Use whole-cell lysates from B-cell lines (e.g., Ramos, Daudi) or tissues rich in FOXP1 (spleen, lung) as positive controls. Validate with knockdown samples if available.
• For immunohistochemistry (IHC-P), antigen retrieval with citrate buffer (pH 6.0) is recommended. Test on formalin-fixed paraffin-embedded human tonsil or spleen sections where nuclear staining is expected.
• In immunofluorescence (IF/ICC), permeabilize cells with 0.1% Triton X-100 and expect nuclear localization. Co-stain with a nuclear marker (e.g., DAPI) to confirm.
• For ELISA, consider using the antibody as a capture or detection reagent in sandwich ELISA; pair with a different clone or anti-FOXP1 polyclonal antibody for optimal sensitivity.
• The immunogen corresponds to amino acids 550-677, which lies C-terminal to the forkhead domain and may recognize multiple isoforms. Verify isoform specificity in your system using isoform-specific controls.
• The antibody cross-reacts with mouse and rat FOXP1, making it suitable for rodent studies. However, confirm reactivity in your specific tissues or cell lines before quantitative assays.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with a one-stop sourcing solution for IVD raw materials, including high-quality monoclonal antibodies like this anti-FOXP1 clone. Our supply chain covers validated antibody pairs, optimized bulk reagents, and technical support, enabling efficient assay development for transcription factor targets and oncology research. Contact us to explore custom sourcing for rare targets or ancillary reagents to accelerate your project from concept to clinic.