Anti-FOXO3A Rabbit Monoclonal Antibody for WB, ELISA - O43524

Core Product Specifications and Parameters

Biological Background: FOXO3A Function and Localization

• FOXO3A is a forkhead box O family transcriptional activator that binds DNA motif 5'-[AG]TAAA[TC]A-3', regulating apoptosis and autophagy. Related references: PMID:10102273 PMID:16751106 PMID:21329882
• In starved conditions, it translocates to the nucleus and induces autophagy gene expression (e.g., GABARAP1L, MAP1LC3B, ATG12), promoting muscle protein breakdown.
• Phosphorylation by MAPKAPK5 enables FOXO3A to upregulate miR-34b/c, which in turn repress MYC translation. Related references: PMID:21329882
• Upon metabolic stress, FOXO3A localizes to mitochondria and enhances mtDNA transcription. Related references: PMID:23283301
• It is ubiquitously expressed and shuttles between cytoplasm and nucleus, with additional mitochondrial matrix and outer membrane localization.
• FOXO3A is a key regulator of Treg differentiation via FOXP3 activation. Related references: PMID:30513302
• Post-translational modifications—including phosphorylation, acetylation, methylation, and ubiquitination—modulate its activity, stability, and subcellular distribution.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide from the C-terminal region (aa 574–673), which may target specific isoforms. Verify reactivity with your sample.
• For WB, the predicted molecular weight is ~71 kDa; consider appropriate controls to confirm specificity, especially given potential post-translational modifications.
• ELISA can be used for quantitative detection; optimize coating and detection conditions for your assay format.
• Since FOXO3A exhibits multiple subcellular localizations, fractionation or immunofluorescence may be necessary to study context-dependent translocation.

CamelBio: Your One-Stop Sourcing Bridge

As a dedicated IVD raw-material partner, CamelBio offers FOXO3A research reagents, validated antibody pairs, and custom monoclonal/polyclonal antibodies for apoptosis and transcription regulation studies. Our one-stop sourcing model also supplies bulk ancillary reagents and rare target antibodies to accelerate your assay development from concept to clinic.