Anti-FOXO3A Polyclonal Antibody for WB, IF/ICC, IF-P, ELISA - Q9WVH4

Core Product Specifications and Parameters

Biological Background: FOXO3A Function and Localization

• Forkhead box protein O3 (FOXO3A), also known as Fkhr2 or Foxo3a, is encoded by the Foxo3 gene.
• FOXO3A is a transcriptional activator that binds the consensus DNA sequence 5'-[AG]TAAA[TC]A-3', regulating apoptosis and autophagy (PubMed:18054315, PubMed:18054316, PubMed:23805378). Related references: PMID:18054315 PMID:18054316 PMID:23805378
• It positively regulates autophagy in skeletal muscle by dephosphorylation, nuclear entry, and activation of autophagy genes like GABARAP1L and MAP1LC3B, leading to proteolysis (PubMed:18054315, PubMed:18054316, PubMed:25402684). Related references: PMID:18054315 PMID:18054316 PMID:25402684
• In response to metabolic stress, FOXO3A translocates to mitochondria to promote mtDNA transcription (PubMed:23283301). Related references: PMID:23283301
• The protein shuttles between the cytoplasm, nucleus, and mitochondria (matrix and outer membrane), with localization changes depending on cellular conditions.
• Tissue expression includes white and brown adipose tissues, liver, kidney, lung, colon, and skeletal muscle (PubMed:22510882, PubMed:29445193). Related references: PMID:22510882 PMID:29445193
• Post-translational modifications include phosphorylation, acetylation, methylation, and ubiquitination, which regulate its activity and stability.
• Additionally, FOXO3A participates in post-transcriptional regulation of MYC via miR-34b/c, promotes chondrogenic commitment of skeletal progenitors (PubMed:32103177), and drives regulatory T-cell differentiation by activating FOXP3. Related references: PMID:32103177

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide spanning amino acids 371–470 of mouse FOXO3A; the resulting polyclonal antibodies may recognize multiple epitopes, making them well-suited for denaturing Western blotting where linearized epitopes are exposed.
• For WB, expect a band at approximately 71 kDa; however, phosphorylation and other modifications may shift or create additional bands, so validate with appropriate controls and phosphatase inhibitors if needed.
• For IF/ICC and IF-P, optimize fixation and permeabilization conditions to ensure antibody access to nuclear and cytoplasmic compartments where FOXO3A localizes.
• For ELISA, this antibody can be considered as either a capture or detection reagent, but users should perform checkerboard titrations to determine optimal working concentrations.
• Cross-reactivity has been confirmed with mouse and rat; reactions with other species should be experimentally verified.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, research labs, and institutes with a comprehensive sourcing solution for FOXO3A-related research and assay development. From validated antibody pairs and bulk polyclonal antibodies to ancillary reagents and rare target raw materials, we offer one-stop access to the tools needed for studying apoptosis, autophagy, and metabolic regulation. Our supply capabilities cover everything from concept to clinic, ensuring reliable and scalable sourcing for your IVD projects.