Anti-FOXO1/FOXO3A/FOXO4 Polyclonal Antibody for WB, IHC-P, ELISA - Q12778 / O43524 / P98177

Core Product Specifications and Parameters

Biological Background: FOXO1/FOXO3A/FOXO4 Function and Localization

• FOXO1 is a forkhead box (FOX) transcription factor that serves as the primary target of insulin signaling, regulating metabolic homeostasis in response to oxidative stress. Related references: PMID:10358076, PMID:12228231, PMID:15220471
• It binds to the insulin response element (IRE) and Daf-16 family binding element (DBE) in DNA, and its transcriptional activity is suppressed by insulin. Related references: PMID:10358076
• FOXO1 is a critical regulator of apoptosis, promoting cell death in various contexts, including neural cells. Related references: PMID:18356527, PMID:19221179
• It is required for autophagic cell death induction in response to starvation or oxidative stress. Related references: PMID:20543840
• In hepatocytes, FOXO1 promotes gluconeogenesis by directly regulating expression of key enzymes such as G6PC1. Related references: PMID:17024043
• FOXO1 shuttles between the cytoplasm and nucleus and is abundantly expressed in skeletal muscle and ovary, with lower expression in many other tissues. Related references: PMID:9479491
• Post-translational modifications including phosphorylation, acetylation, methylation, and ubiquitination tightly regulate FOXO1 activity, localization, and stability.
• This antibody, raised against a synthetic peptide of human FOXO1, also detects the related transcription factors FOXO3A and FOXO4 due to sequence conservation among FOXO family members.

Experimental Guidance and Technical Tips

• Use this polyclonal antibody for Western blotting (WB), immunohistochemistry on paraffin-embedded sections (IHC-P), and ELISA as indicated by the supplier. The diverse epitope recognition of a polyclonal antibody may provide robust signal across these applications.
• The immunogen is a synthetic peptide; consider validating specificity for each FOXO paralog in the relevant sample system, as cross-reactivity has been reported for human, mouse, and rat.
• For WB, FOXO1 has a predicted molecular weight of approximately 70 kDa; bands corresponding to FOXO3A and FOXO4 may appear at similar sizes. Optimize loading controls and blocking conditions for clear resolution.
• In IHC-P, perform antigen retrieval and titrate the antibody to minimize background; nuclear and cytoplasmic staining may be observed depending on cellular context.
• For ELISA, the polyclonal nature allows sensitive detection, but include a defined standard curve and consider paired antibody sets for quantitative assays.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with reliable access to high-quality IVD raw materials, including validated antibody pairs, optimized polyclonal antibodies, bulk ancillary reagents, and custom sourcing for rare targets. This anti-FOXO1/FOXO3A/FOXO4 antibody supports research into metabolic regulation, apoptosis, and transcription factor signaling, with procurement options tailored to your assay development needs. From discovery through to clinical application, CamelBio streamlines your raw material supply chain for diagnostic excellence.