Anti-FOXO1 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - Q12778

Core Product Specifications and Parameters

Biological Background: FOXO1 Function and Localization

• FOXO1 (Forkhead box protein O1, also known as FKHR or FOXO1A) is a transcription factor of the forkhead box family, with a molecular weight of approximately 70 kDa (predicted 69.7 kDa).
• It acts as the main target of insulin signaling, regulating metabolic homeostasis in response to oxidative stress (PubMed:10358076, PubMed:12228231, PubMed:15220471). Related references: PMID:10358076 PMID:12228231 PMID:15220471
• FOXO1 binds to the insulin response element (IRE) and Daf-16 family binding element (DBE) to modulate target gene transcription, and its activity is suppressed by insulin (PubMed:10358076). Related references: PMID:10358076
• Key functions include promoting gluconeogenesis in hepatocytes via cooperation with PPARGC1A and CEBPA (PubMed:17024043), controlling redox balance and bone mass, and regulating osteoblast numbers and chondrogenic commitment (By similarity). Related references: PMID:17024043
• FOXO1 is an important regulator of cell death, promoting apoptosis in neural cells (PubMed:18356527), cardiac smooth muscle cells (PubMed:19483080), and autophagy-mediated cell death (PubMed:20543840). Related references: PMID:18356527 PMID:19483080 PMID:20543840
• Subcellular localization is dynamic: FOXO1 shuttles between the cytoplasm and nucleus in response to post-translational modifications such as phosphorylation and acetylation.
• It is abundantly expressed in skeletal muscle and ovary, with lower levels in heart, placenta, lung, liver, pancreas, spleen, testis, and small intestine, and weak expression in brain, thymus, prostate, and colon (PubMed:9479491). Related references: PMID:9479491
• FOXO1 is subject to multiple post-translational modifications, including acetylation, phosphorylation, methylation, and ubiquitination, which modulate its transcriptional activity and stability.

Experimental Guidance and Technical Tips

• The immunogen encompasses amino acids 340–580 within the C-terminal regulatory region of FOXO1, which contains critical phosphorylation and acetylation sites; thus, this antibody may detect both total and modified forms. Consider validation with phosphatase or deacetylase treatment when assessing specific modification states.
• For Western blot (WB), a band at ~70 kDa is expected; include positive controls such as HeLa, HepG2, or skeletal muscle lysates, as FOXO1 is strongly expressed in these tissues.
• In immunofluorescence/immunocytochemistry (IF/ICC), FOXO1 localizes to both cytoplasm and nucleus, with nuclear translocation promoted by oxidative stress or serum starvation. Optimize fixation and permeabilization conditions to preserve endogenous localization.
• For ELISA, consider pairing with a detection antibody targeting a distinct epitope to enhance specificity; confirm performance using recombinant FOXO1 protein.
• As a rabbit polyclonal antibody, lot-to-lot variability may occur; independent validation with siRNA-mediated knockdown or a blocking peptide is recommended for critical experiments.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with streamlined access to premium IVD raw materials, including this high-quality anti-FOXO1 antibody for metabolism and apoptosis studies. Our comprehensive portfolio spans validated antibody pairs, optimized polyclonal and monoclonal antibodies, and bulk ancillary reagents. For custom requirements or rare targets, our sourcing network ensures reliable supply from concept to clinic.