Anti-FOXN3 Polyclonal Antibody for WB and ELISA - O00409

Core Product Specifications and Parameters

Biological Background: FOXN3 Function and Localization

• FOXN3, also known as checkpoint suppressor 1 (CHES1), is a forkhead box protein that functions as a transcriptional repressor.
• It plays a role in DNA damage response by potentially mediating cell cycle arrests at checkpoints, contributing to genomic stability.
• The protein localizes to the nucleus, consistent with its role in transcription regulation.
• FOXN3 is subject to phosphorylation, indicating regulation via post-translational modifications.
• Alternative splicing produces multiple isoforms, expanding its regulatory potential.

Experimental Guidance and Technical Tips

• For Western blot (WB), consider the predicted molecular weight of approximately 54 kDa and validate using appropriate cell or tissue lysates expressing FOXN3.
• Given the immunogen covers amino acids 269-468, the antibody recognizes a central region of the protein, which may be suitable for detecting multiple isoforms.
• For ELISA, optimize coating antigen and antibody dilution to achieve optimal signal-to-noise ratio.
• Cross-reactivity with mouse allows potential use in murine model studies; however, validation in the relevant sample system is recommended.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. For researchers studying cell cycle regulation and DNA damage checkpoints, CamelBio offers a comprehensive portfolio of validated antibody pairs, optimized polyclonal antibodies like this anti-FOXN3 antibody, and ancillary reagents to support your assay development. Additionally, CamelBio can source rare target raw materials to accelerate your research and diagnostic projects.