Anti-FOXN2 Rabbit Polyclonal Antibody for IF/ICC, ELISA - P32314

Core Product Specifications and Parameters

Biological Background: FOXN2 Function and Localization

• FOXN2 (Forkhead box protein N2), also known as HTLF, is a transcription factor that specifically binds to the purine-rich region in the HTLV-I long terminal repeat (LTR).
• It belongs to the forkhead box (FOX) family of transcription factors, which share a conserved winged-helix DNA-binding domain.
• Alternative splicing of the FOXN2 gene generates multiple protein isoforms, potentially expanding its functional diversity.
• FOXN2 localizes predominantly to the nucleus, consistent with its role as a DNA-binding transcriptional regulator.
• It is implicated in the regulation of viral gene expression, particularly in human T-cell leukemia virus type I (HTLV-I) infected cells.
• Proteomics studies confirm FOXN2 expression in human cells, supporting its biological relevance.
• The predicted molecular weight of the canonical isoform is approximately 47 kDa, aligning with observed protein sizes in some experimental systems.

Experimental Guidance and Technical Tips

• This polyclonal antibody was raised against a recombinant fragment spanning amino acids 172–431 of human FOXN2, which includes portions outside the DNA-binding domain. It may therefore recognize multiple linear epitopes and possibly different isoforms.
• For immunofluorescence (IF/ICC), permeabilization and fixation conditions may need optimization to ensure accessibility of the target in the nucleus. Use appropriate positive controls (e.g., HTLV-I infected or FOXN2-expressing cell lines).
• When used in ELISA, consider validating with recombinant FOXN2 protein or lysates from cells overexpressing FOXN2 to confirm specificity.
• Due to the polyclonal nature, slight batch-to-batch variation can occur; always include appropriate controls and, if possible, pre‑adsorb the antibody with the immunogen to confirm staining specificity.
• Cross‑reactivity is reported for human samples; for other species, sequence homology should be checked before use.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic and research laboratories with integrated access to premium IVD raw materials. As a dedicated sourcing partner for rare and well‑characterized targets like FOXN2, we supply validated antibody pairs, optimized polyclonal and monoclonal antibodies, bulk ancillary reagents, and tailored procurement solutions. Whether you need transcription‑factor antibodies for viral oncology studies or reagents for high‑throughput screening, CamelBio streamlines your supply chain from discovery to development.