Anti-FOXN2 Rabbit Monoclonal Antibody for WB, ELISA - P32314

Core Product Specifications and Parameters

Biological Background: FOXN2 Function and Localization

• FOXN2 (Forkhead box protein N2), also known as Human T-cell leukemia virus enhancer factor (HTLF), is a member of the forkhead box (FOX) family of transcription factors characterized by a conserved DNA-binding domain.
• FOXN2 binds to the purine-rich region in the long terminal repeat (LTR) of human T-cell leukemia virus type I (HTLV-I), thereby acting as a transcriptional regulator.
• The gene is subject to alternative splicing, yielding multiple protein isoforms that may have distinct regulatory functions.
• Subcellular localization is predominantly nuclear, consistent with its role in transcription regulation.
• FOXN2 is identified by proteomics in various human reference proteome datasets, confirming its expression across tissues.
• Post-translational modifications have not been extensively characterized, but the protein’s function in HTLV-I LTR binding suggests involvement in viral latency and leukemogenesis.

Experimental Guidance and Technical Tips

• The immunogen consists of a recombinant fusion protein spanning amino acids 204-431 of FOXN2, which includes part of the forkhead domain. This design may enable detection of both full-length FOXN2 and certain splice variants.
• For Western blot (WB), consider using whole-cell or nuclear extracts from human samples; the expected band is at approximately 47 kDa. KO validation confirms antibody specificity, but titrate and optimize conditions in your own system.
• In ELISA, this antibody may serve as a capture or detection reagent for FOXN2; validate coating and detection protocols with recombinant FOXN2 or cell lysates.
• Cross-reactivity with other species is not reported; limit usage to human samples unless specificity is independently verified.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers, labs, and research institutes with a comprehensive portfolio of IVD raw materials and tailored sourcing services. For FOXN2-related studies—such as HTLV-I transcriptional regulation or leukemia research—we offer validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing. With our one-stop supply model from concept to clinic, you can accelerate assay development and secure high-quality raw materials efficiently.