Anti-FOXL2 Monoclonal Antibody for WB, ELISA - P58012

Core Product Specifications and Parameters

Biological Background: FOXL2 Function and Localization

• FOXL2 is a forkhead box transcription factor essential for ovarian differentiation and maintenance, preventing trans-differentiation of ovary to testis.
• Acts as a transcriptional repressor of the Sertoli cell-promoting gene SOX9, thereby maintaining the ovarian phenotype.
• Modulates transcription of key genes: activates SIRT1 under stress and OSR2; represses STAR; participates in SMAD3-dependent FST transcription.
• Localizes to the nucleus, consistent with its role as a DNA-binding transcription factor.
• Expressed early in somatic cells of the developing gonad and persists in follicular cells of the adult ovary; also expressed in developing eyelid.
• Post-translational modifications include phosphorylation, isopeptide bond formation, and ubiquitin-like conjugation, regulating its activity and stability.
• Mutations in FOXL2 are associated with blepharophimosis-ptosis-epicanthus inversus syndrome (BPES) and premature ovarian failure (POF), highlighting its clinical relevance in reproductive disorders.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide spanning the N-terminal region (amino acids 1–100) of human FOXL2; epitope specificity may influence detection of isoforms or post-translationally modified forms.
• For Western blot, consider using whole-cell lysates from FOXL2-expressing tissues (e.g., granulosa cells, ovarian samples) and validate the predicted band at 39 kDa.
• Cross-reactivity with mouse and rat suggests suitability for orthologous studies, but antibody performance should be confirmed in the relevant species and sample type.
• For ELISA applications, confirm peptide-binding specificity and optimize assay conditions; the antibody may be used for direct or sandwich formats depending on epitope accessibility.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio empowers diagnostic developers and researchers with seamless access to high-quality IVD raw materials. This FOXL2 monoclonal antibody supports studies in reproductive biology and transcription regulation. Beyond single reagents, we offer validated antibody pairs, optimized bulk antibodies, ancillary reagents, and rare target sourcing to accelerate your assay development pipeline.