Anti-FOXK2 Rabbit Polyclonal Antibody for WB and ELISA - Q01167

Core Product Specifications and Parameters

Biological Background: FOXK2 Function and Localization

• FOXK2 encodes a forkhead box transcription factor with multiple isoforms produced by alternative splicing; also known as ILF, ILF1, and nGTBP.
• Recognizes the forkhead motif 5'-GTAAACA-3' and acts as a context-dependent transcriptional activator or repressor (PubMed:22083952, PubMed:25451922). Additionally binds NFAT-like motifs in the IL2 promoter (PubMed:1339390) and the HIV-1 long terminal repeat (PubMed:1909027). Related references: PMID:22083952, PMID:25451922, PMID:1339390, PMID:1909027
• Plays a key role in metabolic reprogramming toward aerobic glycolysis by inducing glycolytic enzymes (HK2, PKLR, LDHA) and PDK1/4, while suppressing mitochondrial oxidation (By similarity).
• Negatively regulates autophagy in skeletal muscle upon starvation by repressing autophagy gene expression (By similarity).
• Positively regulates WNT/β-catenin signaling by mediating nuclear translocation of DVL proteins (PubMed:25805136). Related references: PMID:25805136
• Functions as an accessory component of the polycomb repressive deubiquitinase (PR-DUB) complex, recruiting it to specific target genes (PubMed:24634419, PubMed:30664650). Related references: PMID:24634419, PMID:30664650
• Exhibits G/T-mismatch binding activity with a minimal heteroduplex sequence 5'-TRT[G/T]NB-3' (PubMed:20097901). Related references: PMID:20097901
• Predominantly localizes to the nucleus but is also found in the cytoplasm; expressed broadly in lymphoid and non-lymphoid cells. Post-translationally modified by acetylation, methylation, phosphorylation, and ubiquitination.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 360–430 of human FOXK2; this acidic region may yield antibodies recognizing both native and denatured forms. This antibody is supplied as a rabbit polyclonal and has been validated for Western blotting (WB) and ELISA.
• For WB, expect a band at approximately 69 kDa. Due to the polyclonal nature, additional bands may appear; validate expression in relevant cell or tissue lysates with appropriate controls.
• The antibody is reported to cross-react with human, mouse, and rat FOXK2; verify reactivity in your experimental system.
• For ELISA applications, consider pairing with a capture/detection antibody pair; this pAb may be suitable for direct or indirect assays.
• To ensure reproducibility, titrate the antibody concentration and include recommended blocking agents.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with streamlined access to IVD raw materials, including validated antibody pairs, optimized polyclonal and monoclonal antibodies, bulk ancillary reagents, and rare-target raw-material sourcing. This anti-FOXK2 polyclonal antibody supports research into transcriptional and metabolic regulation, autophagy, and Wnt signaling. For projects requiring complementary reagents—such as FOXK2 monoclonal antibodies, recombinant proteins, or ELISA development kits—our team can assist with sourcing and customized supply solutions.