Anti-FOXK1 Rabbit Polyclonal Antibody for WB, ELISA - P85037

Core Product Specifications and Parameters

Biological Background: FOXK1 Function and Localization

• FOXK1 (Forkhead box protein K1, also known as MNF) is a transcription factor that binds the forkhead DNA motif (5'-GTAAACA-3') and can act as an activator or repressor.
  Related references: PMID:17670796
• It regulates aerobic glycolysis by inducing glycolytic enzymes (HK2, PKLR) and suppressing mitochondrial pyruvate oxidation via PDK1/PDK4.
• FOXK1 promotes muscle progenitor cell proliferation and inhibits myogenic differentiation by repressing FOXO4 and MEFC activity.
• It positively regulates Wnt/β-catenin signaling by translocating DVL into the nucleus.
  Related references: PMID:25805136
• Exhibits antiviral activity by binding ISRE to promote antiviral gene expression.
  Related references: PMID:25852164
• Acts as an accessory component of the PR-DUB deubiquitinase complex, recruiting it to specific genes.
  Related references: PMID:24634419 PMID:30664650
• Phosphorylation by PRKCB isoform β-II promotes ferroptosis via LGALS13 expression and secretion.
  Related references: PMID:40246981
• Subcellular location: primarily nuclear, also cytoplasmic. Expressed in developing and adult tissues, with significant expression in brain, colon, and lymph node tumors. Numerous post-translational modifications include acetylation, methylation, and ubiquitination.

Experimental Guidance and Technical Tips

• The immunogen covers the C-terminal region (aa 600-733) of human FOXK1; the antibody may recognize both full-length and potential splice variants.
• For Western blotting, the predicted molecular weight is ~75 kDa. Validate with positive and negative cell lysates, and consider using loading controls.
• For ELISA, appropriate antigen coating and blocking conditions should be optimized; include known positive and negative controls.
• Since cross-reactivity is reported for human, perform species-specific validation if used in other species.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a one-stop provider of IVD raw materials, supporting diagnostic manufacturers and research labs. For FOXK1-related studies in metabolism, muscle biology, or transcriptional regulation, we offer validated antibodies, recombinant proteins, and ancillary reagents to streamline your assay development. Contact us for bulk sourcing and customized services.