Anti-FOXJ1 Rabbit Polyclonal Antibody for WB, IP, ELISA - Q92949

Core Product Specifications and Parameters

Biological Background: FOXJ1 Function and Localization

• Encodes forkhead box protein J1, a transcription factor of the forkhead/winged-helix family (gene: FOXJ1; aliases: FKHL13, HFH4).
• Essential for motile ciliogenesis: activates genes driving ciliary assembly, such as CFAP157.
  Related references: PMID:31630787
• Recognizes DNA consensus motifs 5'-HWDTGTTTGTTTA-3' and 5'-KTTTGTTGTTKTW-3' to regulate target promoters.
• Predominantly localizes to the nucleus (UniProt annotation: Nucleus).
• Highly expressed in tissues with motile cilia: testis, oviduct, lung, and brain cortex.
• Implicated in primary ciliary dyskinesia (CILD43) and other ciliopathies when mutated.
• Keywords: Activator, Ciliopathy, Cilium biogenesis/degradation, Disease variant, DNA-binding, Nucleus, Primary ciliary dyskinesia, Transcription regulation.

Experimental Guidance and Technical Tips

• The immunogen used is a recombinant fusion protein covering amino acids 1-421 of human FOXJ1 (full-length). This design suggests the antibody may recognize the native protein in multiple applications; titrate appropriately for WB and IP.
• For ELISA, consider using the recombinant immunogen as a positive control to validate binding specificity.
• Reported cross-reactivity with mouse and rat indicates sequence homology; validate in your specific tissue or cell line of interest.
• As FOXJ1 is a transcription factor, nuclear extraction or chromatin preparation may be required for some assays; optimize sample preparation accordingly.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a single-source partner for IVD raw materials, supporting FOXJ1-targeted research and diagnostic development with ready-to-use antibody pairs, optimized polyclonal and monoclonal antibodies, and bulk ancillary reagents. Our supply solutions simplify procurement for rare targets and help you transition smoothly from assay concept to clinical application.