Anti-FOXD1 Rabbit Polyclonal Antibody for WB, ELISA - Q16676

Core Product Specifications and Parameters

Biological Background: FOXD1 Function and Localization

• FOXD1 (Forkhead box protein D1), also known as FKHL8 or FREAC-4, belongs to the forkhead/winged-helix family of transcription factors characterized by a conserved DNA-binding domain.
• It functions as a transcriptional activator, regulating gene expression critical for developmental processes including retinal differentiation, optic chiasm regionalization, kidney morphogenesis, and neuralization of ectodermal cells (By similarity).
• FOXD1 directly activates the transcription of PGF (placental growth factor) and C3 (complement component 3) genes, as demonstrated in cellular studies. Related references: PMID:27805902
• The protein localizes exclusively to the nucleus, consistent with its DNA-binding and transcriptional regulatory role.
• With a calculated molecular weight of approximately 46 kDa (UniProt: 46140 Da), FOXD1 matches the reported protein weight and is classified under keywords such as DNA-binding, transcription regulation, and reference proteome.

Experimental Guidance and Technical Tips

• The immunogen for this antibody is a synthetic peptide derived from the N-terminal region (amino acids 1-100) of human FOXD1. This region is conserved in mouse and rat, supporting the reported cross-reactivity. Consider sequence alignment to confirm epitope conservation in your target species.
• For Western blotting, FOXD1 is expected to migrate at ~46 kDa. Use nuclear-enriched lysates from cell lines or tissues where FOXD1 is expressed (e.g., kidney, retina) to enhance detection.
• In ELISA applications, the peptide immunogen may serve as a positive control for antibody binding. Validate the antibody with recombinant FOXD1 protein or peptide to assess sensitivity and specificity.
• Since FOXD1 is a transcription factor, consider incorporating appropriate controls (e.g., knockdown or knockout samples) to confirm band identity in WB experiments.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic and research communities by supplying high-quality IVD raw materials like this anti-FOXD1 polyclonal antibody, ideal for investigating transcription factor roles in development and disease. We offer a comprehensive range of validated antibodies, recombinant proteins, and ancillary reagents to streamline your assay development. With one-stop sourcing and technical consultation, CamelBio helps you move from concept to clinic efficiently.