Anti-FOXC1 Polyclonal Antibody for WB, ELISA - Q12948

Core Product Specifications and Parameters

Biological Background: FOXC1 Function and Localization

• FOXC1 (Forkhead box protein C1) is a member of the forkhead family of transcription factors, also known as FKHL7 and FREAC-3, characterized by a conserved forkhead DNA-binding domain.
• FOXC1 functions as a DNA-binding transcriptional activator or repressor, recognizing the consensus sequence 5'-[G/C][A/T]AAA[T/C]AA[A/C]-3' and inducing DNA bending upon binding. Related references: PMID:11782474 PMID:12533514 PMID:14506133 PMID:7957066
• It plays critical roles in embryonic development, including eye, bone, cardiovascular, kidney, and skin formation, and acts as a transcriptional coactivator for GLI2 in hedgehog signaling during endochondral ossification. Related references: PMID:11782474 PMID:14578375 PMID:26565916
• FOXC1 regulates FOXO1 expression through promoter binding, thereby influencing cell viability and resistance to oxidative stress, particularly in ocular tissues. Related references: PMID:17993506
• It is involved in cell cycle control by promoting G1 phase arrest via TGFB1-mediated signals, suggesting tumor suppressor activity. Related references: PMID:12408963
• Additionally, FOXC1 promotes epithelial-mesenchymal transition (EMT), cell proliferation, migration, and invasion, and is highly expressed in basal-like breast cancer and hepatocellular tumors. Related references: PMID:20406990 PMID:22991501
• FOXC1 is essential for epidermal keratinocyte terminal differentiation and is expressed in the nucleus of keratinocytes, microvascular invasion formations, and hematopoietic cells. Related references: PMID:27907090 PMID:8499623

Experimental Guidance and Technical Tips

• The immunogen covers the C-terminal region (aa 404-553) of human FOXC1, which may be useful for detecting both full-length and potential C-terminal variants; however, users should verify with appropriate controls.
• For Western blot, the predicted molecular weight is approximately 57 kDa; consider optimizing primary antibody dilution (e.g., 1:500-1:2000) and using lysates from cells known to express FOXC1 (e.g., breast cancer, keratinocyte lines).
• For ELISA, ensure the coating antigen includes or overlaps with the immunogen region to maximize detection sensitivity.
• Cross-reactivity with mouse and rat is predicted based on sequence similarity; validate in the relevant species model if needed.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive portfolio of IVD raw materials, including validated antibodies, antigens, and ancillary reagents for transcription factor and developmental biology research. For targets like FOXC1, our sourcing network can supply optimized polyclonal antibodies, bulk quantities, and rare-target raw materials to support your diagnostic development from concept to clinic. Partner with CamelBio for reliable, one-stop access to critical immunoassay components.